电化学法在有序介孔碳修饰玻碳电极上测定吗啡。

Electrochemical determination of morphine at ordered mesoporous carbon modified glassy carbon electrode.

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied, Chemistry, Graduate University of the Chinese Academy of Sciences, Chinese, Academy of Sciences, Changchun, PR China.

出版信息

Biosens Bioelectron. 2010 Feb 15;25(6):1408-13. doi: 10.1016/j.bios.2009.10.037. Epub 2009 Nov 4.

DOI:10.1016/j.bios.2009.10.037

PMID:19932610

Abstract

A sensitive electrochemical detection scheme for morphine has been proposed by utilizing the ordered mesoporous carbon modified glassy carbon electrode (OMC/GCE). The electrochemical behaviour of morphine at the OMC/GCE was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 82mV) and an obvious increase in the peak current (80 times) compared to the bare glassy carbon electrode (GCE). The results indicated that OMC/GCE remarkably enhanced electrocatalytic activity towards the oxidation of morphine. Linear response was found over the range from 0.1 microM to 20 microM with a high sensitivity of 1.74 microA/microM and low detection limit of 10 nM (S/N=3). And the relative standard deviation was 0.86% (n=5). Moreover, combining with a medium-exchange procedure, the OMC/GCE has also been successfully applied to the selective determination of morphine in urine samples with a low detection limit of 50 nM and satisfied recovery of 96.4%. The good results indicate that the OMC/GCE holds great promise in practical application.

摘要

一种基于有序介孔碳修饰玻碳电极（OMC/GCE）的灵敏电化学检测方案被提出用于检测吗啡。通过循环伏安法研究了吗啡在 OMC/GCE 上的电化学行为。与裸玻碳电极（GCE）相比，修饰电极的过电势（约 82mV）降低，峰电流（80 倍）明显增加。结果表明，OMC/GCE 显著提高了吗啡氧化的电催化活性。在 0.1μM 至 20μM 的范围内发现了线性响应，具有 1.74μA/μM 的高灵敏度和 10 nM（S/N=3）的低检测限。相对标准偏差为 0.86%（n=5）。此外，结合介质交换程序，该 OMC/GCE 还成功应用于尿液样品中吗啡的选择性测定，检测限低至 50 nM，回收率为 96.4%。良好的结果表明，OMC/GCE 在实际应用中具有很大的应用前景。