Department of Food and Nutrition, College of Human Ecology, Hanyang University, Seoul 133-791, South Korea.
Nutr Res. 2009 Nov;29(11):812-8. doi: 10.1016/j.nutres.2009.10.019.
Intake of n-3 polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been suggested to associate with an increased risk of hemorrhagic stroke. The present study was designed to investigate the hypothesis that EPA and DHA increase oxidative stress and hemorrhage volume in rats with intracerebral hemorrhagic (ICH) stroke. Thirty-five-week-old male rats were fed an American Institute of Nutrition-93M diet containing 0% (n = 27), 0.5% (n = 15), or 1% EPA + DHA of total energy for 5 weeks. Of 5 rats fed 1% EPA + DHA (41%), 5 died because of excessive bleeding within 12 hours after ICH surgery. Behavior test score and hemorrhage volume were significantly (P < .05) greater in the 1% EPA + DHA-fed rats than in other rats. Magnetic resonance imaging consistently showed that edema and bleeding were visible in only the rats fed 1% EPA + DHA. Levels of superoxide dismutase and glutathione were significantly (P < .05) lower in rats fed 0.5% and 1% EPA + DHA than those fed 0% EPA + DHA. Thiobarbituric acid-reactive substance content was significantly (P < .05) higher in 1% EPA + DHA-fed rats than in 0% and 0.5% EPA + DHA-fed rats. The level of 8-hydroxydeoxyguanosine was significantly (P < .05) higher in ICH rats with all diets than in sham surgery rats. Brain levels of EPA and DHA were highest in rats fed 1% EPA + DHA than in rats fed 0% and 0.5% EPA + DHA. These results suggested that intake of 1% EPA + DHA of total energy could lead to oxidative damage to the brain and thus increase the risk of intracerebral hemorrhagic stroke in this rat model.
摄入 n-3 多不饱和脂肪酸，如二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），与出血性中风的风险增加有关。本研究旨在验证以下假设，即 EPA 和 DHA 会增加脑出血（ICH）大鼠的氧化应激和出血体积。35 周龄雄性大鼠喂食美国营养学会-93M 饮食，其中 0%（n=27）、0.5%（n=15）或 1%的 EPA+DHA 占总能量的 5 周。在喂食 1%EPA+DHA（41%）的 5 只大鼠中，有 5 只大鼠在 ICH 手术后 12 小时内因过度出血而死亡。与其他大鼠相比，1%EPA+DHA 喂养的大鼠行为测试评分和出血体积明显更大（P<0.05）。磁共振成像一致显示，仅喂食 1%EPA+DHA 的大鼠可见水肿和出血。与喂食 0%EPA+DHA 的大鼠相比，喂食 0.5%和 1%EPA+DHA 的大鼠超氧化物歧化酶和谷胱甘肽水平明显降低（P<0.05）。喂食 1%EPA+DHA 的大鼠丙二醛含量明显高于喂食 0%和 0.5%EPA+DHA 的大鼠（P<0.05）。与假手术大鼠相比，所有饮食组 ICH 大鼠的 8-羟基脱氧鸟苷水平均明显升高（P<0.05）。喂食 1%EPA+DHA 的大鼠脑内 EPA 和 DHA 水平明显高于喂食 0%和 0.5%EPA+DHA 的大鼠。这些结果表明，摄入 1%的 EPA+DHA 占总能量可能导致大脑氧化损伤，从而增加这种大鼠脑出血模型的出血性中风风险。
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