在重组大肠杆菌中甘油到聚（3-羟基丙酸）的转化。
Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli.
机构信息
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany.
出版信息
Appl Environ Microbiol. 2010 Jan;76(2):622-6. doi: 10.1128/AEM.02097-09. Epub 2009 Nov 20.
Abstract
We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.
摘要
我们已经开发出了将甘油转化为热塑性聚（3-羟基丙酸）[聚（3HP）]的方法。为此，我们在重组大肠杆菌中表达了丁酸梭菌的甘油脱水酶（dhaB1）、鼠伤寒沙门氏菌 LT2 的丙醛脱氢酶（pduP）和 Ralstonia eutropha 的聚羟基烷酸（PHA）合酶（phaC1）基因。在两步补料分批发酵中，聚（3HP）的积累量高达 11.98%（wt/wt[细胞干重]）。本研究展示了一种有趣的应用，即在细菌中构建聚（3HP）合成途径。
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