Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China; State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3483-3490. doi: 10.1099/ijsem.0.004202.
Two Gram-stain-negative, short rod-shaped and non-flagellated strains, designated 17-4A and L52-1-41, were isolated from the surface seawater of the Indian Ocean and South China Sea, respectively. The 16S rRNA genes of the two strains shared sequence similarity of 99.45 %. Strain 17-4A shared the highest 16S rRNA gene similarity of 98.02 % with EBR-8-1, followed by BN9 (97.47 %), MJ07 (96.93 %), Ch07 (96.68 %), DCY25 (96.65 %), PB3-7B (96.63 %), 24 (96.34 %), 54Pin (96.28 %) and B201 (96.05 %). L52-1-41 shared the highest 16S rRNA gene similarity of 97.74 % with EBR-8-1, followed by BN9 (97.47 %), MJ07 (96.65 %), Ch07 (96.41 %), DCY25 (96.37 %), PB3-7B (96.35 %), B201 (96.28 %), and 24 (96.06 %). The results of phylogenetic analyses indicated that 17-4A and L52-1-41 formed a stable, distinct and highly supported lineage affiliated to the genus . The results of the digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses indicated that they represented a single species. They featured similar genomic DNA G+C contents of 53.2-53.4 mol%. Activities of catalase and oxidase were negative for both strains. The fatty acids patterns of 17-4A and L52-1-41 were most similar, mostly comprised of C, Ccyclo, C, Cω9 and summed feature 8 (Cω7 and/or C ω6). The major polar lipids of the two strains were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The respiratory quinone of the two strains was Q-8. Hence, on the basis of the phenotypic, chemotaxonomic and genotypic data presented in this study, we proposed the classification of both strains as representatives of a novel species named sp. nov., with the type strain 17-4A (=MCCC 1A12670=KCTC 62121=NBRC 113794), and another strain L52-1-41 (=MCCC 1A05046=KCTC 52313).
两株革兰氏阴性、短杆状且无鞭毛的菌株，分别命名为 17-4A 和 L52-1-41，分别从印度洋和南海的表层海水中分离得到。两株菌的 16S rRNA 基因序列相似性为 99.45%。菌株 17-4A 与 EBR-8-1 的 16S rRNA 基因相似性最高，为 98.02%，其次是 BN9（97.47%）、MJ07（96.93%）、Ch07（96.68%）、DCY25（96.65%）、PB3-7B（96.63%）、24（96.34%）、54Pin（96.28%）和 B201（96.05%）。L52-1-41 与 EBR-8-1 的 16S rRNA 基因相似性最高，为 97.74%，其次是 BN9（97.47%）、MJ07（96.65%）、Ch07（96.41%）、DCY25（96.37%）、PB3-7B（96.35%）、B201（96.28%）和 24（96.06%）。系统发育分析结果表明，17-4A 和 L52-1-41 形成了一个稳定的、独特的、高度支持的属 属谱系。DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）分析结果表明，它们代表了一个单一的物种。它们的基因组 DNA G+C 含量相似，为 53.2-53.4mol%。两株菌的过氧化氢酶和氧化酶活性均为阴性。17-4A 和 L52-1-41 的脂肪酸图谱最为相似，主要由 C、Ccyclo、C、Cω9 和特征性 8（Cω7 和/或 C ω6）组成。两株菌的主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和未鉴定的胺脂。两株菌的呼吸醌均为 Q-8。因此，基于本研究中表型、化学分类和基因型数据，我们提出将这两株菌分类为一个新种，命名为 sp. nov.，其模式菌株为 17-4A（=MCCC 1A12670=KCTC 62121=NBRC 113794）和另一个菌株 L52-1-41（=MCCC 1A05046=KCTC 52313）。
