Millet P, Fisk T L, Collins W E, Broderson J R, Nguyen-Dinh P
Malaria Branch, Center for Infectious Diseases, U.S. Department of Health and Human Services, Atlanta, Georgia 30333.
Am J Trop Med Hyg. 1988 Dec;39(6):529-34. doi: 10.4269/ajtmh.1988.39.529.
Exoerythrocytic stage parasites of Plasmodium cynomolgi, P. knowlesi, P. coatneyi, and P. inui were cultured by inoculating primary cultures of hepatocytes from Macaca mulatta with sporozoites. Less than 1% of inoculated sporozoites survived. Morphology and size of the liver stages in all 4 species were similar to in vivo descriptions and the time required for in vitro maturation correlated well with prepatent periods described.
通过用食蟹猴疟原虫、诺氏疟原虫、科氏疟原虫和猪尾疟原虫的子孢子接种恒河猴肝细胞的原代培养物，培养了这些疟原虫的红细胞外期寄生虫。接种的子孢子存活率不到1%。所有这4种疟原虫肝期的形态和大小与体内描述相似，体外成熟所需时间与所述的潜伏期相关性良好。
