• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在伤口愈合过程中调节视网膜转录组的基因组位点。

Genomic loci modulating the retinal transcriptome in wound healing.

作者信息

Vázquez-Chona Félix R, Lu Lu, Williams Robert W, Geisert Eldon E

机构信息

Moran Eye Center, University of Utah, Salt Lake City, UT 84132, USA.

出版信息

Gene Regul Syst Bio. 2008 Feb 14;1:327-48.

PMID:19936100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2759132/
Abstract

PURPOSE

The present study predicts and tests genetic networks that modulate gene expression during the retinal wound-healing response.

METHODS

Upstream modulators and target genes were defined using meta-analysis and bioinformatic approaches. Quantitative trait loci (QTLs) for retinal acute phase genes (Vazquez-Chona et al. 2005) were defined using QTL analysis of CNS gene expression (Chesler et al. 2005). Candidate modulators were defined using computational analysis of gene and motif sequences. The effect of candidate genes on wound healing was tested using animal models of gene expression.

RESULTS

A network of early wound-healing genes is modulated by a locus on chromosome 12. The genetic background of the locus altered the wound-healing response of the retina. The C57BL/6 allele conferred enhanced expression of neuronal marker Thy1 and heat-shock-like crystallins, whereas the DBA/2J allele correlated with greater levels of the classic marker of retinal stress, glial fibrillary acidic protein (GFAP). Id2 and Lpin1 are candidate upstream modulators as they strongly correlated with the segregation of DBA/2J and C57BL/6 alleles, and their dosage levels correlated with the enhanced expression of survival genes (Thy1 and crystallin genes).

CONCLUSION

We defined a genetic network associated with the retinal acute injury response. Using genetic linkage analysis of natural transcript variation, we identified regulatory loci and can didate modulators that control transcript levels of acute phase genes. Our results support the convergence of gene expression profiling, QTL analysis, and bioinformatics as a rational approach to discover molecular pathways controlling retinal wound healing.

摘要

目的

本研究预测并测试在视网膜伤口愈合反应过程中调节基因表达的遗传网络。

方法

使用荟萃分析和生物信息学方法定义上游调节因子和靶基因。通过对中枢神经系统基因表达进行数量性状基因座（QTL）分析（Chesler等人，2005年）来定义视网膜急性期基因的QTL（Vazquez-Chona等人，2005年）。使用基因和基序序列的计算分析来定义候选调节因子。使用基因表达动物模型测试候选基因对伤口愈合的影响。

结果

一个早期伤口愈合基因网络受12号染色体上一个位点的调节。该位点的遗传背景改变了视网膜的伤口愈合反应。C57BL/6等位基因使神经元标志物Thy1和热休克样晶状体蛋白的表达增强，而DBA/2J等位基因与视网膜应激经典标志物胶质纤维酸性蛋白（GFAP）的更高水平相关。Id2和Lpin1是候选上游调节因子，因为它们与DBA/2J和C57BL/6等位基因的分离密切相关，并且它们的剂量水平与存活基因（Thy1和晶状体蛋白基因）的增强表达相关。

结论

我们定义了一个与视网膜急性损伤反应相关的遗传网络。通过对自然转录本变异进行遗传连锁分析，我们鉴定了控制急性期基因转录水平的调控位点和候选调节因子。我们的结果支持将基因表达谱分析、QTL分析和生物信息学相结合，作为发现控制视网膜伤口愈合分子途径的合理方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/6c665a37608f/grsb-2007-327f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/40d328c2571e/grsb-2007-327f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/d50e0a18a7ca/grsb-2007-327f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/e54a12f2abd2/grsb-2007-327f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/b081039ed0da/grsb-2007-327f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/6c665a37608f/grsb-2007-327f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/40d328c2571e/grsb-2007-327f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/d50e0a18a7ca/grsb-2007-327f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/e54a12f2abd2/grsb-2007-327f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/b081039ed0da/grsb-2007-327f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30b6/2759132/6c665a37608f/grsb-2007-327f5.jpg

相似文献

1
Genomic loci modulating the retinal transcriptome in wound healing.在伤口愈合过程中调节视网膜转录组的基因组位点。
Gene Regul Syst Bio. 2008 Feb 14;1:327-48.
2
Genetic networks controlling retinal injury.控制视网膜损伤的基因网络。
Mol Vis. 2005 Nov 4;11:958-70.
3
Quantitative genetics of age-related retinal degeneration: a second F1 intercross between the A/J and C57BL/6 strains.年龄相关性视网膜变性的数量遗传学：A/J和C57BL/6品系之间的第二次F1杂交
Mol Vis. 2007 Jan 25;13:79-85.
4
The abundance of cis-acting loci leading to differential allele expression in F1 mice and their relationship to loci harboring genes affecting complex traits.导致F1小鼠中差异等位基因表达的顺式作用位点的丰度及其与携带影响复杂性状基因的位点的关系。
BMC Genomics. 2016 Aug 11;17(1):620. doi: 10.1186/s12864-016-2922-9.
5
Using gene expression databases for classical trait QTL candidate gene discovery in the BXD recombinant inbred genetic reference population: mouse forebrain weight.利用基因表达数据库在BXD重组近交遗传参考群体中发现经典性状QTL候选基因：小鼠前脑重量
BMC Genomics. 2008 Sep 25;9:444. doi: 10.1186/1471-2164-9-444.
6
Genomic locus modulating corneal thickness in the mouse identifies POU6F2 as a potential risk of developing glaucoma.调控小鼠角膜厚度的基因组位点将 POU6F2 鉴定为潜在的青光眼发病风险因素。
PLoS Genet. 2018 Jan 25;14(1):e1007145. doi: 10.1371/journal.pgen.1007145. eCollection 2018 Jan.
7
New quantitative trait loci that regulate wound healing in an intercross progeny from DBA/1J and 129 x 1/SvJ inbred strains of mice.调控DBA/1J和129×1/SvJ近交系小鼠杂交后代伤口愈合的新数量性状基因座。
Funct Integr Genomics. 2006 Apr;6(2):157-63. doi: 10.1007/s10142-005-0004-1. Epub 2005 Oct 6.
8
Microarray analysis of retinal gene expression in the DBA/2J model of glaucoma.青光眼DBA/2J模型中视网膜基因表达的微阵列分析。
Invest Ophthalmol Vis Sci. 2006 Mar;47(3):977-85. doi: 10.1167/iovs.05-0865.
9
Application of quantitative trait locus mapping and transcriptomics to studies of the senescence-accelerated phenotype in rats.数量性状基因座定位和转录组学在大鼠衰老加速表型研究中的应用。
BMC Genomics. 2014;15 Suppl 12(Suppl 12):S3. doi: 10.1186/1471-2164-15-S12-S3. Epub 2014 Dec 19.
10
A strong and highly significant QTL on chromosome 6 that protects the mouse from age-related retinal degeneration.位于6号染色体上的一个强大且高度显著的数量性状基因座，可保护小鼠免受与年龄相关的视网膜退化。
Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2442-9. doi: 10.1167/iovs.02-1252.

引用本文的文献

1
Robust classification of wound healing stages in both mice and humans for acute and burn wounds based on transcriptomic data.基于转录组数据对急性和烧伤伤口的小鼠和人类进行稳健的伤口愈合阶段分类。
BMC Bioinformatics. 2023 Apr 25;24(1):166. doi: 10.1186/s12859-023-05295-z.
2
Using BXD mouse strains in vision research: A systems genetics approach.利用 BXD 鼠系进行视觉研究：一种系统遗传学方法。
Mol Vis. 2020 Mar 6;26:173-187. eCollection 2020.
3
Enhancing the efficacy of AREDS antioxidants in light-induced retinal degeneration.提高年龄相关性眼病研究组（AREDS）抗氧化剂在光诱导视网膜变性中的疗效。

本文引用的文献

1
Conditional ablation of Stat3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury.Stat3或Socs3的条件性消融揭示了脊髓损伤后反应性星形胶质细胞的双重作用。
Nat Med. 2006 Jul;12(7):829-34. doi: 10.1038/nm1425. Epub 2006 Jun 18.
2
Genetic networks controlling retinal injury.控制视网膜损伤的基因网络。
Mol Vis. 2005 Nov 4;11:958-70.
3
Mechanistic insights into glaucoma provided by experimental genetics the cogan lecture.实验遗传学对青光眼的机制性见解——科根讲座
Mol Vis. 2017 Oct 10;23:718-739. eCollection 2017.
4
A practical approach to optic nerve crush in the mouse.一种针对小鼠视神经挤压伤的实用方法。
Mol Vis. 2012;18:2147-52. Epub 2012 Jul 27.
5
Association of high myopia with crystallin beta A4 (CRYBA4) gene polymorphisms in the linkage-identified MYP6 locus.CRYBA4 基因多态性与连锁定位的 MYP6 基因座高度近视的相关性。
PLoS One. 2012;7(6):e40238. doi: 10.1371/journal.pone.0040238. Epub 2012 Jun 29.
6
Networks modulating the retinal response to injury: insights from microarrays, expression genetics, and bioinformatics.调节视网膜对损伤反应的网络：来自微阵列、表达遗传学和生物信息学的见解。
Adv Exp Med Biol. 2012;723:649-56. doi: 10.1007/978-1-4614-0631-0_82.
7
Functional rescue of experimental ischemic optic neuropathy with αB-crystallin.用 αB-晶状体蛋白对实验性缺血性视神经病变的功能挽救。
Eye (Lond). 2011 Jun;25(6):809-17. doi: 10.1038/eye.2011.42. Epub 2011 Apr 8.
8
Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush.C57BL/6J小鼠和DBA/2J小鼠对视神经挤压的不同反应。
BMC Neurosci. 2009 Jul 30;10:90. doi: 10.1186/1471-2202-10-90.
Invest Ophthalmol Vis Sci. 2005 Aug;46(8):2649-61. doi: 10.1167/iovs.05-0205.
4
WebGestalt: an integrated system for exploring gene sets in various biological contexts.WebGestalt：一个用于在各种生物学背景下探索基因集的集成系统。
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W741-8. doi: 10.1093/nar/gki475.
5
The genomic response to retinal disease and injury: evidence for endothelin signaling from photoreceptors to glia.视网膜疾病和损伤的基因组反应：从光感受器到神经胶质细胞的内皮素信号传导证据。
J Neurosci. 2005 May 4;25(18):4540-9. doi: 10.1523/JNEUROSCI.0492-05.2005.
6
Mapping expression in randomized rodent genomes.绘制随机啮齿动物基因组中的表达图谱。
Nat Genet. 2005 Mar;37(3):209-10. doi: 10.1038/ng0305-209.
7
Uncovering regulatory pathways that affect hematopoietic stem cell function using 'genetical genomics'.利用“遗传基因组学”揭示影响造血干细胞功能的调控途径。
Nat Genet. 2005 Mar;37(3):225-32. doi: 10.1038/ng1497. Epub 2005 Feb 13.
8
Complex trait analysis of gene expression uncovers polygenic and pleiotropic networks that modulate nervous system function.基因表达的复杂性状分析揭示了调节神经系统功能的多基因和多效性网络。
Nat Genet. 2005 Mar;37(3):233-42. doi: 10.1038/ng1518. Epub 2005 Feb 13.
9
Expression of acute-phase response proteins in retinal Müller cells in diabetes.糖尿病状态下视网膜Müller细胞中急性期反应蛋白的表达
Invest Ophthalmol Vis Sci. 2005 Jan;46(1):349-57. doi: 10.1167/iovs.04-0860.
10
Content-rich biological network constructed by mining PubMed abstracts.通过挖掘PubMed摘要构建的内容丰富的生物网络。
BMC Bioinformatics. 2004 Oct 8;5:147. doi: 10.1186/1471-2105-5-147.