Biological Sciences, Michigan Technological University, Houghton, MI, USA.
Curr Microbiol. 2010 Apr;60(4):294-300. doi: 10.1007/s00284-009-9540-6. Epub 2009 Nov 19.
Isolate RS1(T) isolated from used metalworking fluid was found to be a Gram-negative, motile, and non-spore forming rod. Based on phylogenetic analyses with 16S rRNA, isolate RS1(T) was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C(18:1)omega7c (32.6%), C(16:0) (25.5%), and C(15:0) ISO 2OH/C(16:1)omega7c (14.4%). The sequence similarities of isolate RS1(T) based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062(T) and 0.63 with P. pseudoalcaligenes ATCC17440(T). The DNA G + C content of isolate RS1(T) was 62.2 mol.%. The DNA-DNA relatedness was 73.0% with P. oleovorans ATCC 8062(T) and 79.1% with P. pseudoalcaligenes ATCC 17440(T). On the basis of morphological, biochemical, and molecular studies, isolate RS1(T) is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA-DNA relatedness (>70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440(T) and P. oleovorans ATCC 8062(T) should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062(T) = DSM 1045(T) = NCIB 6576(T)), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1(T) = ATCC BAA-1494(T) = DSM 21016(T)).
从使用过的金属加工液中分离出的 RS1(T) 被鉴定为革兰氏阴性、可运动、非孢子形成的杆菌。根据 16S rRNA 的系统发育分析，分离株 RS1(T) 被归入假单胞菌属的门多萨亚属。主要的全细胞脂肪酸为 C(18:1)ω7c（32.6%）、C(16:0)（25.5%）和 C(15:0) ISO 2OH/C(16:1)ω7c（14.4%）。基于 gyrB 和 rpoD 基因的 RS1(T) 序列相似性与 Pseudomonas pseudoalcaligenes 分别为 98.9%和 98.0%，与 Pseudomonas oleovorans 分别为 98.5%和 98.1%。核糖体分型图谱与 Pseudomonas oleovorans ATCC 8062(T) 的相似度为 0.60，与 Pseudomonas pseudoalcaligenes ATCC 17440(T) 的相似度为 0.63。分离株 RS1(T) 的 DNA G+C 含量为 62.2mol%。与 Pseudomonas oleovorans ATCC 8062(T) 的 DNA-DNA 相关性为 73.0%，与 Pseudomonas pseudoalcaligenes ATCC 17440(T) 的 DNA-DNA 相关性为 79.1%。基于形态学、生化和分子研究，分离株 RS1(T) 被认为代表了 Pseudomonas oleovorans 的一个新亚种。此外，根据 DNA-DNA 相关性（>70%）、化学分类学和分子特征，Pseudomonas pseudoalcaligenes ATCC 17440(T) 和 Pseudomonas oleovorans ATCC 8062(T) 应归为同一名称；根据优先权规则，首先描述的物种 Pseudomonas oleovorans 是较早的同义词，而 Pseudomonas pseudoalcaligenes 是后来的同义词。因此，建议将 Pseudomonas oleovorans 物种划分为两个新亚种：Pseudomonas oleovorans subsp. oleovorans 亚种。nov.（模式株 ATCC 8062(T)=DSM 1045(T)=NCIB 6576(T)），Pseudomonas oleovorans subsp. lubricantis 亚种。nov.（模式株 RS1(T)=ATCC BAA-1494(T)=DSM 21016(T)）。
