古管假单胞菌新种，一种从温泉中分离到的γ-变形菌。
Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring.
机构信息
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan, ROC.
出版信息
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4591-4598. doi: 10.1099/ijs.0.047712-0. Epub 2013 Aug 5.
An aerobic, Gram-stain-negative, rod-shaped bacterium (designated strain CC-G9A(T)), motile by a polar-flagellum, was isolated from a hot spring water sample in Taiwan. Strain CC-G9A(T) could grow at 20-42 °C, pH 6.0-10.0 and tolerate up to 7% (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-G9A(T) showed pairwise sequence similarity to Pseudomonas mendocina LMG 1223(T) (97.7%), Pseudomonas alcaligenes ATCC 14909(T) (97.8 %), Pseudomonas alcaliphila DSM 17744(T) (97.8 %), Pseudomonas toyotomiensis JCM 15604(T) (97.6 %), Pseudomonas oleovorans subsp. lubricantis DSM 21016(T) (97.6 %) and Pseudomonas argentinensis BCRC 17807(T) (97.5 %), and lower sequence similarity to other species of the genus Pseudomonas. According to DNA-DNA association analysis, the relatedness of strain CC-G9A(T) to P. mendocina BCRC 10458(T), P. alcaliphila DSM 17744(T), P. alcaligenes BCRC 11893(T), P. oleovorans subsp. lubricantis DSM 21016(T), P. argentinensis BCRC 17807(T) and P. oleovorans subsp. oleovorans BCRC 11902 was 55.1±3.1, 13.7±1.5, 14.1±1.8, 58.5±1.1, 28.9±2.0 and 28.6±1.8 %, respectively. The evolutionary trees reconstructed based on 16S rRNA, gyrB and rpoB gene sequences revealed varying phylogenetic neighbourhoods of strain CC-G9A(T) with regard to the most closely related type strains. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 64.3±1.3 mol%. The major fatty acids were C10 : 0 3-OH, C12 : 0, C12 : 0 3-OH, C16 : 0 and summed features 3 and 8 consisting of C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-G9A(T) is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas guguanensis sp. nov. is proposed. The type strain is CC-G9A(T) ( = BCRC 80438(T) = JCM 18416(T)).
一株好氧、革兰氏阴性、杆状细菌（命名为 CC-G9A(T) 菌株），通过极生鞭毛运动，从台湾温泉水样中分离得到。CC-G9A(T) 菌株可在 20-42°C、pH6.0-10.0 和耐受高达 7%（w/v）NaCl 的条件下生长。CC-G9A(T) 菌株的 16S rRNA 基因序列分析显示与 Pseudomonas mendocina LMG 1223(T)（97.7%）、Pseudomonas alcaligenes ATCC 14909(T)（97.8%）、Pseudomonas alcaliphila DSM 17744(T)（97.8%）、Pseudomonas toyotomiensis JCM 15604(T)（97.6%）、Pseudomonas oleovorans subsp. lubricantis DSM 21016(T)（97.6%）和 Pseudomonas argentinensis BCRC 17807(T)（97.5%）具有较高的序列相似性，而与其他假单胞菌属的种则具有较低的序列相似性。根据 DNA-DNA 同源性分析，CC-G9A(T) 菌株与 P. mendocina BCRC 10458(T)、P. alcaliphila DSM 17744(T)、P. alcaligenes BCRC 11893(T)、P. oleovorans subsp. lubricantis DSM 21016(T)、P. argentinensis BCRC 17807(T)和 P. oleovorans subsp. oleovorans BCRC 11902 的相关度分别为 55.1±3.1、13.7±1.5、14.1±1.8、58.5±1.1、28.9±2.0 和 28.6±1.8%。基于 16S rRNA、gyrB 和 rpoB 基因序列构建的进化树显示，CC-G9A(T) 菌株与最密切相关的模式菌株在系统发育上存在不同的亲缘关系。主要醌类为泛醌 9（Q-9），DNA G+C 含量为 64.3±1.3 mol%。主要脂肪酸为 C10:0 3-OH、C12:0、C12:0 3-OH、C16:0 和由 C16:1ω7c/C16:1ω6c 和 C18:1ω7c/C18:1ω6c 组成的特征脂肪酸 3 和 8。主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰甘油。根据独特的系统发育、表型和化学分类特征，CC-G9A(T) 菌株被提议代表假单胞菌属中的一个新种，命名为 Pseudomonas guguanensis sp. nov.。其模式菌株为 CC-G9A(T)（=BCRC 80438(T)=JCM 18416(T)）。
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