Department of Comparative Genetics and Refinement, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ, Rijswijk, The Netherlands.
Immunogenetics. 2009 Dec;61(11-12):745-53. doi: 10.1007/s00251-009-0412-9. Epub 2009 Nov 24.
The major histocompatibility complex (MHC) class I B gene/allelic repertoire was investigated in a pedigreed population of cynomolgus macaques of mixed Indonesian/Malaysian origin. The Mafa-B alleles detected in this cohort are mostly specific for a given geographic area, and only a small number of alleles appears to be shared with other populations. This suggests the fast evolution of Mafa-B alleles due to adaptation to new environments. In contrast to humans, the B locus in Old World monkeys displays extensive copy number variation. The Mafa-B and previously defined -A gene combinations segregate in families and thus allowed the definition of extended haplotypes. In many cases it was possible to assign a particular Mafa-I allele to one of these Mafa-A/B haplotypes as well. The presence of a large number of stable haplotypes in this cohort of animals, which was pedigreed for up to eight generations, looks promising for developing discriminative MHC typing tools that are less cumbersome. Furthermore, the discovery of 53 unreported Mafa-B sequences expands the lexicon of alleles significantly, and may help in understanding the complex organisation of the macaque B region.
本研究调查了源自印度尼西亚/马来西亚混合起源的食蟹猴 pedigreed 群体中的主要组织相容性复合体（MHC）I 类 B 基因/等位基因库。在该队列中检测到的 Mafa-B 等位基因主要特定于特定地理区域，只有少数等位基因似乎与其他群体共享。这表明 Mafa-B 等位基因由于适应新环境而快速进化。与人类不同，旧世界猴的 B 基因座显示出广泛的拷贝数变异。Mafa-B 和之前定义的 -A 基因组合在家族中分离，因此允许定义扩展的单倍型。在许多情况下，也可以将特定的 Mafa-I 等位基因分配给这些 Mafa-A/B 单倍型之一。在这群动物中，存在大量稳定的单倍型，这些动物已经进行了多达八代的 pedigreed 研究，这为开发不那么繁琐的有区别的 MHC 分型工具带来了希望。此外，发现了 53 个未报告的 Mafa-B 序列，这极大地扩展了等位基因的词汇量，并可能有助于理解猕猴 B 区的复杂组织。
