黄芩苷对重症急性胰腺炎大鼠胰腺组织中肿瘤坏死因子-α mRNA、半胱天冬酶-3及P-选择素表达的影响
Influence of baicalin on TNF-alpha mRNA, caspase-3 and P-selectin expression in pancreatic tissue of rats with severe acute pancreatitis.
作者信息
Xiping Zhang, Hua Tian, Jie Zhang, Li Chen, Yang Cai, Bei Lu, Qijun Yang, Jing Ye, Wengqin Yuan, Ninnin Zhou
机构信息
Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China.
出版信息
Indian J Gastroenterol. 2009 Jul-Aug;28(4):131-5. doi: 10.1007/s12664-009-0047-7. Epub 2009 Nov 24.
OBJECTIVE
Baicalin reduces the severity of severe acute pancreatitis (SAP) in a rat model. This study was carried out to examine the effect of baicalin on TNF-alpha mRNA, caspase-3 and P-selectin protein expression in the pancreas of rats with SAP.
METHODS
Rats with SAP were randomly assigned to untreated, baicalin-treated, octreotide-treated and sham-operated group. The TNF-alpha mRNA expression level, caspase-3 and P-selectin protein expression levels of pancreatic tissue were measured 3, 6 and 12 hours after operation.
RESULTS
The caspase-3 protein expression level was significantly higher in the treated groups than that in the untreated group at 3 hours (p <0.01). P-selectin protein expression level in pancreas of the treated groups was significantly lower than that of the untreated group (p <0.05 or <0.01). TNF-alpha mRNA expression level in pancreas decreased in baicalintreated group while untreated group increased at different time points (p <0.05).
CONCLUSION
Baicalin increases caspase-3 expression and inhibits TNF-alpha mRNA and P-selectin protein expression in pancreatic tissue of SAP rats.
目的
黄芩苷可减轻大鼠重症急性胰腺炎（SAP）模型的严重程度。本研究旨在探讨黄芩苷对SAP大鼠胰腺中肿瘤坏死因子-α（TNF-α）mRNA、半胱天冬酶-3（caspase-3）和P-选择素蛋白表达的影响。
方法
将SAP大鼠随机分为未治疗组、黄芩苷治疗组、奥曲肽治疗组和假手术组。在术后3、6和12小时测量胰腺组织中TNF-α mRNA表达水平、caspase-3和P-选择素蛋白表达水平。
结果
术后3小时，治疗组的caspase-3蛋白表达水平显著高于未治疗组（p<0.01）。治疗组胰腺中P-选择素蛋白表达水平显著低于未治疗组（p<0.05或<0.01）。黄芩苷治疗组胰腺中TNF-α mRNA表达水平下降，而未治疗组在不同时间点升高（p<0.05）。
结论
黄芩苷可增加SAP大鼠胰腺组织中caspase-3表达，并抑制TNF-α mRNA和P-选择素蛋白表达。
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