黄芩苷与奥曲肽对重症急性胰腺炎大鼠肝损伤的保护作用研究
Study on protecting effects of baicalin and octreotide on hepatic injury in rats with severe acute pancreatitis.
作者信息
Zhang Xi-Ping, Zhang Jie, Ren Zheng, Feng Guang-Hua, Zhu Wei, Cai Yang, Yang Qi-Jun, Ju Tong-Fa, Xie Qi, Yuan Wen-Qin
机构信息
Department of General Surgery, Hangzhou First People's Hospital, 261 Huansha Road, Hangzhou 310006, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2008 Nov 14;14(42):6551-9. doi: 10.3748/wjg.14.6551.
AIM
To investigate the protective effects and mechanisms of baicalin and octreotide on hepatic injury in rats with severe acute pancreatitis (SAP).
METHODS
The SAP rat models were prepared and randomly assigned to the model control group, baicalin treated group, and octreotide treated group while other healthy rats were assigned to the sham-operated group. Rat mortality, levels of ALT, AST, liver and pancreas pathological changes in all groups were observed at 3, 6 and 12 h after operation. Tissue microarray (TMA) sections of hepatic tissue were prepared to observe expression levels of Bax, Bcl-2 protein and caspase-3, and changes of apoptotic indexes.
RESULTS
Rat survival at 12 h, expression levels of Bax, caspase-3 protein and apoptotic indexes of liver were all significantly higher in treated groups than in model control group. While the liver and pancreas pathological scores, contents of ALT, AST, and expression levels of Bcl-2 protein were all lower in treated groups than in the model control group.
CONCLUSION
Both baicalin and octreotide can protect rats with SAP by decreasing the contents of ALT, AST and expression levels of Bcl-2 protein, and improving the expression levels of Bax protein, caspase-3 protein, and inducing apoptosis.
目的
探讨黄芩苷和奥曲肽对重症急性胰腺炎（SAP）大鼠肝损伤的保护作用及机制。
方法
制备SAP大鼠模型，随机分为模型对照组、黄芩苷治疗组和奥曲肽治疗组，另取健康大鼠作为假手术组。术后3、6和12 h观察各组大鼠死亡率、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）水平、肝脏和胰腺病理变化。制备肝组织组织芯片（TMA）切片，观察Bax、Bcl-2蛋白和半胱天冬酶-3（caspase-3）的表达水平及凋亡指数变化。
结果
治疗组大鼠12 h存活率、肝脏Bax、caspase-3蛋白表达水平及凋亡指数均显著高于模型对照组。而治疗组肝脏和胰腺病理评分、ALT、AST含量及Bcl-2蛋白表达水平均低于模型对照组。
结论
黄芩苷和奥曲肽均可通过降低ALT、AST含量及Bcl-2蛋白表达水平，提高Bax蛋白、caspase-3蛋白表达水平并诱导凋亡来保护SAP大鼠。
Zotero 插件