用于研究蛋白质内在无序性的氢交换质谱法。
Hydrogen-exchange mass spectrometry for the study of intrinsic disorder in proteins.
作者信息
Balasubramaniam Deepa, Komives Elizabeth A
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0378, USA.
出版信息
Biochim Biophys Acta. 2013 Jun;1834(6):1202-9. doi: 10.1016/j.bbapap.2012.10.009. Epub 2012 Oct 22.
Abstract
Amide hydrogen/deuterium exchange detected by mass spectrometry (HXMS) is seeing wider use for the identification of intrinsically disordered parts of proteins. In this review, we discuss examples of how discovery of intrinsically disordered regions and their removal can aid in structure determination, biopharmaceutical quality control, the characterization of how post-translational modifications affect weak structuring of disordered regions, the study of coupled folding and binding, and the characterization of amyloid formation. This article is part of a Special Issue entitled: Mass spectrometry in structural biology.
摘要
通过质谱法检测的酰胺氢/氘交换（HXMS）在鉴定蛋白质内在无序区域方面的应用越来越广泛。在本综述中，我们讨论了一些例子，说明内在无序区域的发现及其去除如何有助于结构测定、生物制药质量控制、翻译后修饰对无序区域弱结构影响的表征、耦合折叠与结合的研究以及淀粉样蛋白形成的表征。本文是名为“结构生物学中的质谱分析”的特刊的一部分。
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