蛋白质构象与动力学研究中的氢/氘交换和质谱分析：是否需要自上而下的方法？
H/D exchange and mass spectrometry in the studies of protein conformation and dynamics: is there a need for a top-down approach?
作者信息
Kaltashov Igor A, Bobst Cedric E, Abzalimov Rinat R
机构信息
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
出版信息
Anal Chem. 2009 Oct 1;81(19):7892-9. doi: 10.1021/ac901366n.
Abstract
Hydrogen/deuterium exchange (HDX) combined with mass spectrometry (MS) detection has matured in recent years to become a powerful tool in structural biology and biophysics. Several limitations of this technique can and will be addressed by tapping into the ever expanding arsenal of methods to manipulate ions in the gas phase offered by mass spectrometry.
摘要
近年来，氢/氘交换（HDX）与质谱（MS）检测相结合已发展成熟，成为结构生物学和生物物理学中的一种强大工具。通过利用质谱在气相中操纵离子的不断扩充的方法库，该技术的一些局限性能够且将会得到解决。
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