Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
Am J Chin Med. 2009;37(6):1203-14. doi: 10.1142/S0192415X09007600.
Resveratrol is a polyphenol compound and prominent anti-inflammatory agent found in plants, including the fruits of Morus alba. However, the therapeutic mechanisms of resveratrol remain largely unclear. To gain insight into the biological effects of resveratrol, we examined its influence on LPS-induced IL-8 production in the human monocytic cell line, THP-1. In inflammatory diseases, IL-8 plays a central role in the initiation and maintenance of inflammatory response. In the present study, IL-8 production was measured by ELISA and RT-PCR, while MAPK activation, IkappaBalpha degradation, nuclear factor (NF)-kappaB activation and cyclooxygenase (COX)-2 expression were determined by Western blot analysis. Resveratrol inhibited LPS-induced IL-8 production in a dose-dependent manner. Furthermore, resveratrol inhibited extracellular signal-regulated kinase (ERK) and p38 MAPK phosphorylation, IkappaBalpha degradation, NF-kappaB activation and cyclooxygenase (COX)-2 expression, which suggest that resveratrol inhibits IL-8 secretion by blocking MAPK phosphorylation and NF-kappaB activation. Taken together, these findings may help elucidate the mechanism by which resveratrol modulates THP-1 cell activation under inflammatory conditions.
白藜芦醇是一种多酚化合物，也是一种主要的抗炎剂，存在于植物中，包括桑科植物的果实。然而，白藜芦醇的治疗机制在很大程度上仍不清楚。为了深入了解白藜芦醇的生物学效应，我们研究了它对人单核细胞系 THP-1 中 LPS 诱导的 IL-8 产生的影响。在炎症性疾病中，IL-8 在炎症反应的启动和维持中起着核心作用。在本研究中，通过 ELISA 和 RT-PCR 测定了 IL-8 的产生，而通过 Western blot 分析测定了 MAPK 激活、IκBα降解、核因子（NF）-κB 激活和环氧化酶（COX）-2 的表达。白藜芦醇呈剂量依赖性抑制 LPS 诱导的 IL-8 产生。此外，白藜芦醇抑制细胞外信号调节激酶（ERK）和 p38 MAPK 磷酸化、IκBα降解、NF-κB 激活和环氧化酶（COX）-2 的表达，这表明白藜芦醇通过阻断 MAPK 磷酸化和 NF-κB 激活抑制 IL-8 的分泌。综上所述，这些发现可能有助于阐明白藜芦醇在炎症条件下调节 THP-1 细胞活化的机制。
