State Key Laboratory for Turbulence and Complex System and Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, No. 5, Yi-He-Yuan Road, Beijing 100871, China.
Biomaterials. 2010 Feb;31(6):1093-103. doi: 10.1016/j.biomaterials.2009.11.015. Epub 2009 Nov 24.
Mg-Zn-Ca bulk metallic glass with different compositions (Mg66Zn30Ca4 and Mg70Zn25Ca5) have been prepared for this study and their feasibility as biodegradable metallic materials have been evaluated by the microstructural, surface analysis, mechanical testing, corrosion and cytotoxicity tests. It was found that the Mg66Zn30Ca4 sample presents a more uniform corrosion morphology than as-rolled pure Mg and Mg70Zn25Ca5 samples, with much smaller micro-scale uniformly distributed pores beneath the corrosion product layer. The corrosion products were identified to be Mg(OH)(2) and Zn(OH)(2), and a uniform corrosion mechanism is proposed. Both indirect cytotoxicity and direct cell culture experiments were carried out using L929 and MG63 cell lines. The results show higher cell viabilities for Mg-Zn-Ca extracts than that for as-rolled pure Mg. In addition, L929 and MG63 cells were found to adhere and proliferate on the surface of Mg66Zn30Ca4 sample.
本研究制备了不同成分的 Mg-Zn-Ca 块体金属玻璃（Mg66Zn30Ca4 和 Mg70Zn25Ca5），通过微观结构、表面分析、力学性能测试、腐蚀和细胞毒性测试评估了它们作为可生物降解金属材料的可行性。结果表明，与轧制态纯镁和 Mg70Zn25Ca5 相比，Mg66Zn30Ca4 样品的腐蚀形貌更均匀，腐蚀产物层下有更小的微尺度均匀分布的微孔。腐蚀产物被鉴定为 Mg(OH)(2)和 Zn(OH)(2)，并提出了一种均匀腐蚀机制。使用 L929 和 MG63 细胞系进行了间接细胞毒性和直接细胞培养实验。结果表明，Mg-Zn-Ca 提取物的细胞活力比轧制态纯镁高。此外，L929 和 MG63 细胞被发现可以在 Mg66Zn30Ca4 样品表面黏附和增殖。
