Laboratory of Environmental Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, People's Republic of China.
Bioresour Technol. 2010 Apr;101(8):2600-7. doi: 10.1016/j.biortech.2009.10.059. Epub 2009 Nov 24.
In this study, the microbial response of anaerobic sludge digestion to the addition of two classic methanogenic inhibitors (chloroform, 2-bromoethanesulfonate) was investigated. Both the toxicants showed their effectiveness on CH(4) production, whereas the hydrogen responses and acetate accumulations were elicited to different extent. Terminal restriction fragment length polymorphism analyses in combination with clone library showed that both toxicants inhibited not only methanogenic activity but the structure of methanogenic communities. The acetoclastic Methanosaetaceae was more sensitive than hydrogenotrophic Methanobacteriales and Methanomicrobiales. Interestingly, as reflected by the favorable thermodynamic condition and the increase of formyltetrahydrofolate synthetase (fhs) gene copy numbers, reductive homoacetogenesis from H(2)/CO(2) was also stimulated by selective inhibition of methanogenesis with 2-bromoethanesulfonate (BES).
在这项研究中，考察了两种经典产甲烷抑制剂（三氯甲烷、2-溴乙磺酸钠）对厌氧污泥消化中微生物反应的影响。这两种毒物都对 CH(4)的产生表现出了抑制作用，而氢的反应和乙酸的积累则受到不同程度的激发。末端限制性片段长度多态性分析与克隆文库相结合的方法表明，这两种毒物不仅抑制了产甲烷活性，还抑制了产甲烷菌群落的结构。与氢营养型的 Methanobacteriales 和 Methanomicrobiales 相比，乙酸营养型的 Methanosaetaceae 更为敏感。有趣的是，正如有利的热力学条件和甲酰四氢叶酸合成酶 (fhs) 基因拷贝数的增加所反映的那样，用 2-溴乙磺酸钠（BES）选择性抑制产甲烷作用也刺激了 H(2)/CO(2)的还原同型产乙酸作用。
