Logroño Washington, Nikolausz Marcell, Harms Hauke, Kleinsteuber Sabine
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, 04318 Leipzig, Germany.
Microorganisms. 2022 Feb 3;10(2):355. doi: 10.3390/microorganisms10020355.
Mixed or pure cultures can be used for biomethanation of hydrogen. Sodium 2-bromoethanesulfonate (BES) is an inhibitor of methanogenesis used to investigate competing reactions like homoacetogenesis in mixed cultures. To understand the effect of BES on the hydrogenotrophic metabolism in a biomethanation process, anaerobic granules from a wastewater treatment plant, a hydrogenotrophic enrichment culture, and pure cultures of and were incubated under H/CO headspace in the presence or absence of BES, and the turnover of H, CO, CH, formate and acetate was analyzed. Anaerobic granules produced the highest amount of formate after 24 h of incubation in the presence of BES. Treating the enrichment culture with BES led to the accumulation of formate. produced more formate than when treated with BES. The non-inhibited methanogenic communities produced small amounts of formate whereas the pure cultures did not. The highest amount of acetate was produced by the anaerobic granules concomitantly with formate consumption. These results indicate that formate is an important intermediate of hydrogenotrophic metabolism accumulating upon methanogenesis inhibition.
混合培养物或纯培养物可用于氢气的生物甲烷化。2-溴乙烷磺酸钠（BES）是一种产甲烷作用抑制剂，用于研究混合培养物中的竞争反应，如同型产乙酸作用。为了解BES对生物甲烷化过程中氢营养代谢的影响，将来自污水处理厂的厌氧颗粒、氢营养富集培养物以及 和 的纯培养物在有或无BES存在的H₂/CO₂顶空条件下进行培养，并分析H₂、CO₂、CH₄、甲酸盐和乙酸盐的转化情况。在有BES存在的情况下培养24小时后，厌氧颗粒产生的甲酸盐量最高。用BES处理富集培养物导致甲酸盐积累。用BES处理时， 产生的甲酸盐比 更多。未受抑制的产甲烷群落产生少量甲酸盐，而纯培养物则不产生。厌氧颗粒伴随着甲酸盐的消耗产生了最高量的乙酸盐。这些结果表明，甲酸盐是氢营养代谢的重要中间产物，在产甲烷作用受到抑制时会积累。
