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Infrared-reflection-mode near-field microscopy using an apertureless probe with a resolution of lambda/600.
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Polarization-selective mapping of near-field intensity and phase around gold nanoparticles using apertureless near-field microscopy.使用无孔径近场显微镜对金纳米颗粒周围的近场强度和相位进行偏振选择性映射。
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Chemical imaging of the surface of self-assembled polystyrene-b-poly(methyl methacrylate) diblock copolymer films using apertureless near-field IR microscopy.使用无孔近场红外显微镜对自组装聚苯乙烯-b-聚（甲基丙烯酸甲酯）二嵌段共聚物薄膜表面进行化学成像。
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Analysis of nano-chemical mapping performed by an AFM-based ("AFMIR") acousto-optic technique.基于原子力显微镜（“AFMIR”）声光技术的纳米化学图谱分析。
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Infrared spectroscopic mapping of single nanoparticles and viruses at nanoscale resolution.纳米级分辨率下单纳米颗粒和病毒的红外光谱映射。
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Force microscopy studies of fibronectin adsorption and subsequent cellular adhesion to substrates with well-defined surface chemistries.纤连蛋白吸附以及随后细胞与具有明确表面化学性质的底物的黏附的力显微镜研究。
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用于蛋白质成像的无孔径近场红外显微镜的组装、调试及使用

Assembly, tuning and use of an apertureless near field infrared microscope for protein imaging.

作者信息

Paulite Melissa, Fakhraai Zahra, Akhremitchev Boris B, Mueller Kerstin, Walker Gilbert C

机构信息

Department of Chemistry, University of Toronto, Toronto, Canada.

出版信息

J Vis Exp. 2009 Nov 25(33):1581. doi: 10.3791/1581.

DOI:10.3791/1581
PMID:19940838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3304400/
Abstract

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit. The apertureless near-field microscope is a light scattering-type instrument that provides infrared spectra at circa 20 nm resolution. A complete list of components and a step-by-step protocol for use is provided. Common errors in assembly and instrument tuning are discussed. A representative data set that shows the secondary structure of an amyloid fibril is presented.

摘要

本文旨在指导读者组装和操作一台用于超越衍射极限成像的红外近场显微镜。无孔径近场显微镜是一种光散射型仪器，能提供约20纳米分辨率的红外光谱。文中提供了完整的组件列表和详细的使用步骤。讨论了组装和仪器调谐过程中常见的错误。展示了一个显示淀粉样原纤维二级结构的代表性数据集。