Institute of Physiological Chemistry, Dresden University of Technology, Fiedlerstrasse 42, 01307, Dresden, Germany.
Glycoconj J. 2010 Jan;27(1):151-8. doi: 10.1007/s10719-009-9270-9. Epub 2009 Nov 26.
Glycosaminoglycans (GAG) and proteoglycans, which are components of the extracellular bone matrix, are also localized in and at the membrane of osteoblasts and in the pericellular matrix. Due to their interaction with several growth factors, water and cations these molecules play an important role in regulating proliferation and differentiation of osteoblasts and bone development. The aim of this study was to assess in vitro the effects of two chemically sulfated hyaluronan (HyaS) derivatives on the proliferation of rat calvarial osteoblasts and to compare with those of native hyaluronan (Hya) and natural sulfated GAG such as chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), dermatan sulfate (DS) and heparan sulfate (HS). Moderately and highly sulfated HyaS derivatives caused a time-dependent reduction of osteoblast proliferation. The anti-proliferative effect of HyaS was accompanied by a cell cycle arrest in the G1 phase, but was not associated with cell death. Whereas non-sulfated high molecular weight (HMW)- and low molecular weight (LMW)-Hya as well as C4S, C6S, DS and HS showed no effect on the cell proliferation.
糖胺聚糖（GAG）和蛋白聚糖是细胞外骨基质的组成部分，也存在于成骨细胞的膜内和细胞周基质中。由于它们与多种生长因子、水和阳离子相互作用，这些分子在调节成骨细胞的增殖和分化以及骨骼发育方面发挥着重要作用。本研究的目的是评估两种化学硫酸化透明质酸（HyaS）衍生物对大鼠颅骨成骨细胞增殖的体外影响，并与天然透明质酸（Hya）以及天然硫酸化 GAG（如硫酸软骨素 4-硫酸盐（C4S）、硫酸软骨素 6-硫酸盐（C6S）、硫酸皮肤素（DS）和硫酸乙酰肝素（HS）进行比较。中度和高度硫酸化的 HyaS 衍生物可导致成骨细胞增殖呈时间依赖性减少。HyaS 的抗增殖作用伴随着细胞周期停滞在 G1 期，但与细胞死亡无关。而非硫酸化的高分子量（HMW）和低分子量（LMW）-Hya 以及 C4S、C6S、DS 和 HS 对细胞增殖没有影响。
