生成稳定的诱导Th1/CTL、Th2和Th17的人树突状细胞。
Generation of stable Th1/CTL-, Th2-, and Th17-inducing human dendritic cells.
作者信息
Kalinski Pawel, Wieckowski Eva, Muthuswamy Ravikumar, de Jong Esther
机构信息
Department of Surgery, University of Pittsburgh and University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA.
出版信息
Methods Mol Biol. 2010;595:117-33. doi: 10.1007/978-1-60761-421-0_7.
Dendritic cells (DC) are the most potent inducers and regulators of immune responses, responsible for communication within immune system. The ability of DC to act both as the inducers of immune responses and as regulatory/suppressive cells led to the interest in their immunotherapeutic use in different disease types, ranging from cancer to autoimmunity, and as a tool to prevent the rejection of transplanted tissues and organs. Over the last years, several groups including ours have demonstrated the feasibility of obtaining monocyte-derived DC with different functions, by modulating the conditions and the duration of DC maturation. The current chapter provides a detailed protocol of generating type-1-, type-2-, and type-17-polarized DC for testing the cytokine-producing abilities of these cells and their effectiveness in inducing Th1, Th2, and Th17 responses of CD4(+) T cells and CTL responses of naïve and memory CD8(+) T cells.
树突状细胞（DC）是免疫反应中最有效的诱导剂和调节剂，负责免疫系统内的通讯。DC既能作为免疫反应的诱导剂，又能作为调节/抑制细胞，这使得人们对其在不同疾病类型（从癌症到自身免疫性疾病）中的免疫治疗应用产生了兴趣，并将其作为预防移植组织和器官排斥反应的工具。在过去几年中，包括我们在内的几个研究小组已经证明，通过调节DC成熟的条件和持续时间，可以获得具有不同功能的单核细胞衍生DC。本章提供了生成1型、2型和17型极化DC的详细方案，用于测试这些细胞产生细胞因子的能力及其在诱导CD4(+) T细胞的Th1、Th2和Th17反应以及幼稚和记忆CD8(+) T细胞的CTL反应方面的有效性。
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