Gazi University, Gazi Education Faculty, Department of Science Education, Teknikokullar, Ankara, Turkey.
Food Chem Toxicol. 2010 Feb;48(2):633-8. doi: 10.1016/j.fct.2009.11.044. Epub 2009 Nov 23.
Mature male Wistar rats (weighing 300-320 g and each group six animals) were given malathion (27 mg/kg; 1/50 of the LD50 for an oral dose), vitamin C (200 mg/kg)+vitamin E (200 mg/kg), or both daily via gavage for 4 weeks. At the end of the fourth week, the malathion-treated group and the malathion plus vitamin-treated group both had significantly higher white blood cell (WBC) and thrombocyte counts than the control group. Compared to the control group, the two groups also had significantly higher serum total cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels, and significantly lower triglyceride and very low density lipoprotein (VLDL) levels. The malathion-treated rats also had significantly lower serum total protein and albumin levels, but the malathion plus vitamin-treated group did not differ from the control group in terms of these parameters. Moreover, concomitant vitamin treatment significantly normalized, at least partially, all of the other hematological and biochemical parameters that were altered by malathion. Light microscopic analyses revealed that both the malathion-treated and malathion plus vitamin-treated groups exhibited histopathological changes in liver tissues, although some pathological features were only observed in the malathion-treated group. Thus, vitamins C and E can reduce malathion hepatotoxicity, although the degree of protection they provide is limited.
成熟雄性 Wistar 大鼠（体重 300-320 g，每组 6 只）经灌胃给予马拉硫磷（27 mg/kg；口服 LD50 的 1/50）、维生素 C（200 mg/kg）+维生素 E（200 mg/kg）或两者每日 1 次，共 4 周。第 4 周末，马拉硫磷处理组和马拉硫磷加维生素处理组的白细胞（WBC）和血小板计数均明显高于对照组。与对照组相比，两组的血清总胆固醇、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH）水平也明显升高，而甘油三酯和极低密度脂蛋白（VLDL）水平明显降低。马拉硫磷处理组的血清总蛋白和白蛋白水平也明显降低，但马拉硫磷加维生素处理组在这些参数上与对照组无差异。此外，维生素的联合治疗至少部分地使马拉硫磷改变的所有其他血液学和生化学参数正常化。光镜分析显示，马拉硫磷处理组和马拉硫磷加维生素处理组的肝脏组织均出现组织病理学变化，尽管一些病理特征仅在马拉硫磷处理组中观察到。因此，维生素 C 和 E 可以减轻马拉硫磷的肝毒性，但它们提供的保护程度有限。
