Shokrzadeh Mohammad, Shobi Sepideh, Attar Hossein, Shayegan Sahel, Payam Sakineh Sadat Hosseini, Ghorbani Faezeh
Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, 18 Kilometer of Farahabad Road, Sari, Iran.
Pak J Biol Sci. 2012 Oct 1;15(19):936-41. doi: 10.3923/pjbs.2012.936.941.
Diazinon, a commonly used organophosphorus pesticide, has been widely used throughout the world in agriculture and horticulture to control insects that feed on crops, ornamentals, lawns, fruits, vegetables and other food products. The toxicity of the DZN causes adverse effects on many organs. The purpose of this study was to examine the protective effect of vitamins A, E and C on liver enzymes alanine transaminase (ALT), Aspartate aminotransferase (AST) and Lactate Dehydrogenase (LDH) in rats exposed to diazinon. In this study, male wistar rats were randomly divided into 10 different groups. The groups were administered normal saline, soybean oil (as the solvent for diazinon and fat-soluble vitamins), diazinon, (30 mg kg(-1), vitamins E, C and A (100, 500 mg kg(-1) and 400 IU kg(-1), respectively) and a combination of diazinon with the same dose of each vitamin intraperitoneally i.p.daily for 14 days. Seven days after the final injection, the animals were anesthetized and blood samples were taken. The photometric method was used to measure the activity of the enzymes. The activities of ALT and AST in the diazinon group were significantly higher than that observed in the control group; however, the diazinon/vitamin E, A, C group displayed significant reduction in ALT and AST activities compared to the diazinon group. The lowest level of LDH enzyme activity was observed in the dazinon/vitamin C group and this was statistically lower than the diazinon group. The results of this study revealed that vitamin E, A and C have a potent protective effect against diazinon-induced hepatotoxicity in rats, which may be due to the scavenging of free radicals and increased antioxidant status.
二嗪农是一种常用的有机磷农药，在全球范围内广泛应用于农业和园艺领域，用于防治以农作物、观赏植物、草坪、水果、蔬菜及其他食品为食的昆虫。二嗪农的毒性会对许多器官产生不良影响。本研究的目的是考察维生素A、E和C对暴露于二嗪农的大鼠肝脏酶丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和乳酸脱氢酶（LDH）的保护作用。在本研究中，雄性Wistar大鼠被随机分为10个不同的组。这些组分别腹腔注射生理盐水、大豆油（作为二嗪农和脂溶性维生素的溶剂）、二嗪农（30 mg kg⁻¹）、维生素E、C和A（分别为100、500 mg kg⁻¹和400 IU kg⁻¹），以及二嗪农与每种维生素相同剂量的组合，每天注射，持续14天。末次注射7天后，将动物麻醉并采集血样。采用光度法测定酶的活性。二嗪农组的ALT和AST活性显著高于对照组；然而，与二嗪农组相比，二嗪农/维生素E、A、C组的ALT和AST活性显著降低。在二嗪农/维生素C组中观察到LDH酶活性最低，且在统计学上低于二嗪农组。本研究结果表明，维生素E、A和C对二嗪农诱导的大鼠肝毒性具有显著的保护作用，这可能是由于清除自由基和提高抗氧化状态所致。
