Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):1-7. doi: 10.1016/j.jchromb.2009.09.033.
The mass fragmentation patterns of stilbene glycosides isolated from the genus Lysidice were investigated by negative ion electrospray ionization tandem mass spectrometry, and the influence of collision energy on their fragmentation behavior is discussed. It is found that the presence of the Y(0)(-) and B(0)(-) ions in the MS(2) spectra is characteristic for 1-->6 linked diglycosyl stilbenes, while the Y(0)(-), Y(1)(-), and Z(1)(-) ions are representative ions of 1-->2 linked diglycosyl stilbenes. These results indicate that ESI-MS(n) in the negative ion mode can be used to differentiate 1-->6 and 1-->2 linked diglycosyl stilbenes. Based on the fragmentation rules, 9 new trace constituents were identified or tentatively characterized in a fraction of Lysidice brevicalyx by using HPLC/HRMS and HPLC-DAD/ESI-MS(n). The results of the present study can assist in on-line structural identification of analogous constituents and targeted isolation of novel compounds from crude plant extracts.
采用负离子电喷雾串联质谱技术研究了从穗花杉属中分离得到的二苯乙烯苷的质谱裂解规律，并探讨了碰撞能对其裂解行为的影响。结果表明，MS2谱中存在 Y(0)(-)和 B(0)(-)离子是 1-->6 连接的二糖基二苯乙烯的特征，而 Y(0)(-)、Y(1)(-)和 Z(1)(-)离子则是 1-->2 连接的二糖基二苯乙烯的代表性离子。这些结果表明，负离子模式下的 ESI-MS(n)可用于区分 1-->6 和 1-->2 连接的二糖基二苯乙烯。基于裂解规律，采用高效液相色谱/高分辨质谱（HPLC/HRMS）和高效液相色谱-二极管阵列检测-电喷雾串联质谱（HPLC-DAD/ESI-MS(n)）在短序穗花杉的一个馏分中鉴定或初步表征了 9 种新的痕量成分。本研究结果有助于对类似成分进行在线结构鉴定，并从粗提物中靶向分离新型化合物。
