INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
Water Res. 2010 Mar;44(6):1873-83. doi: 10.1016/j.watres.2009.11.027. Epub 2009 Nov 20.
The objective of this study was to identify fecal bacteria able to persist after wastewater treatment and that could be used as indicators of human fecal contamination. In a first step, the diversity of Bacteroidales, Clostridiaceae, Bifidobacterium, and Bacillus-Streptococcus-Lactobacillus cluster (BSL) was analysed using a fingerprint technique (CE-SSCP) and 16S rDNA libraries in waters collected at the end of the treatment process in different urban wastewater treatment plants. For each group, dominant bacteria present in most effluents were identified. Their origin (human feces, animal feces, non-fecal) was then analysed based on data of their closest relatives in public 16S rDNA databases. Among fecal bacteria recovered in the treated effluents analysed, phylotypes close to Bifidobacterium adolescentis and Bacteroides caccae seem to be specific to human beings. Phylotypes gathering only sequences of human fecal origin were also identified among the BSL and Clostridiaceae, two bacterial groups which have been poorly investigated for bacterial source-tracking purpose. Since these bacteria were detected post-treatment in most wastewater treatment plants, they may constitute potential new indicators of fecal contamination specific to humans that could be used to track fecal contamination of surface water by sewage.
本研究的目的是鉴定出能在废水处理后仍能存活并可作为人类粪便污染指示物的粪便细菌。在第一步中，采用指纹技术（CE-SSCP）和 16S rDNA 文库，对不同城市污水处理厂处理工艺末端采集的水样中的拟杆菌门（Bacteroidales）、梭菌科（Clostridiaceae）、双歧杆菌（Bifidobacterium）和芽孢杆菌-链球菌-乳杆菌群（BSL）的多样性进行了分析。对于每个群组，都鉴定出了大多数污水中存在的优势细菌。然后根据其在公共 16S rDNA 数据库中最接近的亲缘关系的数据，分析其来源（人类粪便、动物粪便、非粪便）。在所分析的处理污水中回收的粪便细菌中，与青春双歧杆菌（Bifidobacterium adolescentis）和脆弱拟杆菌（Bacteroides caccae）密切相关的菌型似乎是人类特有的。在 BSL 和梭菌科中也鉴定出了仅聚集人类粪便来源序列的菌型，这两个细菌群组在用于细菌溯源的目的时研究甚少。由于这些细菌在大多数污水处理厂的处理后都被检测到，因此它们可能成为特定于人类粪便污染的潜在新型指示物，可用于追踪污水对地表水的粪便污染。
