通过直接测序少量免疫沉淀 DNA 进行染色质分析。
Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
机构信息
Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
出版信息
Nat Methods. 2010 Jan;7(1):47-9. doi: 10.1038/nmeth.1404. Epub 2009 Nov 29.
Abstract
Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
摘要
通过对蛋白质占据或其他特性进行划分的基因组 DNA 进行测序，可以对染色质结构和转录因子定位进行全基因组分析，但目前的技术涉及多个步骤，这些步骤会引入偏差和效率低下的问题。在这里，我们应用单分子方法直接对经过最小样本处理的染色质免疫沉淀 DNA 进行测序。该方法仅需要 50pg 的 DNA，因此应该有助于从有限的细胞群体中绘制染色质图谱。
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