Department of Cancer Biology, Institute of Genetics and Molecular and Cellular Biology, Strasbourg-Illkirch, France.
Nat Methods. 2011 Jun 5;8(7):565-7. doi: 10.1038/nmeth.1626.
Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells.
基于免疫沉淀染色质的大规模平行测序（ChIP-seq）的全基因组转录因子谱分析需要纳克数量的 DNA。在这里，我们描述了一种高保真度、单管线性 DNA 扩增方法（LinDA），可用于 ChIP-seq 和 reChIP-seq，其所需的 DNA 量仅为从几千个细胞中获得的皮克数量。这种扩增技术将促进对非常小的细胞群体（如干细胞或起始癌细胞）中的转录因子结合和染色质进行的全球分析。
