Liu Yang, Zhang Hong, Zhang Luwei, Zhang Xu, Xie Yi, Zhao Weiping
Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Pharmazie. 2009 Oct;64(10):685-9.
The present study was performed to obtain evidence of the radioprotective function of melatonin at different administration levels on carbon ion-induced mouse testicular damage. Outbred Kun-Ming strain mice were divided into six groups, each composed of eight animals: control group, melatonin alone group, irradiation group and three melatonin plus irradiation-treated groups. An acute study was carried out to determine alterations in DNA-single strand break, cell apoptosis, and oxidative stress parameters as well as histopathology in mouse testis 24 h after whole-body irradiation with a single dose of 4 Gy. The results showed that pre-treatment and post-treatment with high-dose melatonin (10 mg/kg) both significantly alleviated carbon ion-induced acute testicular damage, a greater radioprotective effect being observed in the pre-treatment group. On the other hand, low-dose melatonin (1 mg/kg) had a limited radioprotective effect on irradiation-induced degeneration and DNA lesions in mouse testis. Taken together, the data suggest that prophylactic treatment with a higher dose of melatonin is probably advisable to protect against the effects of heavy-ion irradiation.
本研究旨在获得褪黑素在不同给药水平对碳离子诱导的小鼠睾丸损伤的辐射防护功能的证据。远交群昆明品系小鼠分为六组，每组八只动物：对照组、单独使用褪黑素组、照射组和三个褪黑素加照射处理组。进行了一项急性研究，以确定在单次全身照射4 Gy后24小时小鼠睾丸中DNA单链断裂、细胞凋亡、氧化应激参数以及组织病理学的变化。结果表明，高剂量褪黑素（10 mg/kg）预处理和后处理均显著减轻了碳离子诱导的急性睾丸损伤，预处理组观察到更大的辐射防护效果。另一方面，低剂量褪黑素（1 mg/kg）对照射诱导的小鼠睾丸退化和DNA损伤的辐射防护作用有限。综上所述，数据表明，较高剂量的褪黑素预防性治疗可能有助于预防重离子辐射的影响。
