Khitrik N M, Didkovskiĭ N A, Malashenkova I K, Tsurko V V, Egorov I V
Ter Arkh. 2009;81(10):49-52.
To study neutrophil bacterial and absorptive functions and the specific features of their impairments in gout.
The study included 48 male patients with a valid diagnosis of gout (mean age, 59.7 +/- 10.3 years; duration of the disease, 9.2 +/- 2.1 years; blood uric acid (UA), 520 +/- 80 micromol) (Group 1); 25 apparently healthy volunteers (mean age 55.1 +/- 6.2 years; blood UA, 350 micromol/l) (Group 2). Neutrophil functional activity was estimated from the phagocytic-nitroblue tetrazolium reduction (NBT test) and myeloperoxidase (MPO) activity, the determination of non-enzyme cationic proteins (CP) and their spontaneous and induced indices. Neutrophil phagocytic function was also studied.
In Group 1, the content of active oxygen forms (AOF) was increased, on average, to 113.3 +/- 8.65 conventional units (CI) versus 88.22 +/- 5.23 CI in Group 2; p < 0.05). In 34 (70.8%) of the 48 male patients with gout, spontaneous MPO activity was significantly reduced as compared with that in Group 2: 348.4 +/- 13.6 and 509.2 +/- 12.2 CI, respectively (p < 0.01). In Group 1, the level of CP was noticeably decreased to 60.1 +/- 2.06 CI whereas in Group 2, it was 84.91 +/- 5.36 CI (p < 0.05). In gouty patients, the CP stimulation index averaged 0.70 +/- 0.13; in Group 2, it was significantly higher--1.2 +/- 0.05 (p < 0.05).
In the majority of gouty patients, neutrophil functional activity suffers due to its lower phagocytic function, which suppresses the body's antioxidant defense and contributes to the chronic pattern of an inflammatory process.
研究痛风患者中性粒细胞的细菌吞噬和吸收功能及其功能受损的具体特征。
本研究纳入48例确诊痛风的男性患者（平均年龄59.7±10.3岁；病程9.2±2.1年；血尿酸（UA）520±80μmol）（第1组）；25名健康志愿者（平均年龄55.1±6.2岁；血尿酸350μmol/l）（第2组）。通过吞噬-硝基蓝四唑还原试验（NBT试验）和髓过氧化物酶（MPO）活性、非酶阳离子蛋白（CP）的测定及其自发和诱导指数评估中性粒细胞的功能活性。同时研究了中性粒细胞的吞噬功能。
第1组中，活性氧形式（AOF）的含量平均增加至113.3±8.65个常规单位（CI），而第2组为88.22±5.23 CI；p<0.05）。48例痛风男性患者中有34例（70.8%）的自发MPO活性与第2组相比显著降低：分别为348.4±13.6和509.2±12.2 CI（p<0.01）。第1组中，CP水平显著降低至60.1±2.06 CI，而第2组为84.91±5.36 CI（p<0.05）。痛风患者中，CP刺激指数平均为0.70±0.13；第2组显著更高——1.2±0.05（p<0.05）。
在大多数痛风患者中，中性粒细胞的功能活性因吞噬功能降低而受损，这抑制了机体的抗氧化防御并促成了炎症过程的慢性化。
