University of Naples 'Federico II', Dept of Soil, Plant, Environmental and Animal Production Sciences, Via Università 100, 80055 Portici, Italy.
BMC Plant Biol. 2009 Nov 30;9:142. doi: 10.1186/1471-2229-9-142.
Since no genome sequences of solanaceous plants have yet been completed, expressed sequence tag (EST) collections represent a reliable tool for broad sampling of Solanaceae transcriptomes, an attractive route for understanding Solanaceae genome functionality and a powerful reference for the structural annotation of emerging Solanaceae genome sequences.
We describe the SolEST database http://biosrv.cab.unina.it/solestdb which integrates different EST datasets from both cultivated and wild Solanaceae species and from two species of the genus Coffea. Background as well as processed data contained in the database, extensively linked to external related resources, represent an invaluable source of information for these plant families. Two novel features differentiate SolEST from other resources: i) the option of accessing and then visualizing Solanaceae EST/TC alignments along the emerging tomato and potato genome sequences; ii) the opportunity to compare different Solanaceae assemblies generated by diverse research groups in the attempt to address a common complaint in the SOL community.
Different databases have been established worldwide for collecting Solanaceae ESTs and are related in concept, content and utility to the one presented herein. However, the SolEST database has several distinguishing features that make it appealing for the research community and facilitates a "one-stop shop" for the study of Solanaceae transcriptomes.
由于茄科植物的基因组序列尚未完成,表达序列标签(EST)集代表了对茄科转录组进行广泛采样的可靠工具,这是了解茄科基因组功能的一种有吸引力的途径,也是新兴茄科基因组序列结构注释的有力参考。
我们描述了 SolEST 数据库 http://biosrv.cab.unina.it/solestdb,该数据库整合了来自栽培和野生茄科物种以及两个 Coffea 属物种的不同 EST 数据集。数据库中包含的背景和处理后的数据与外部相关资源广泛链接,为这些植物家族提供了宝贵的信息来源。与其他资源相比,SolEST 有两个新功能:i)可以访问并可视化沿着新兴的番茄和马铃薯基因组序列的茄科 EST/TC 比对;ii)可以比较不同研究小组生成的不同茄科组装,以尝试解决 SOL 社区的一个常见问题。
全世界已经建立了不同的数据库来收集茄科 EST,并在概念、内容和用途上与本文提出的数据库相关。然而,SolEST 数据库具有一些区别于其他数据库的特点,使其对研究社区具有吸引力,并为茄科转录组的研究提供了一个“一站式”服务。