State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.
Diagn Microbiol Infect Dis. 2010 Mar;66(3):274-84. doi: 10.1016/j.diagmicrobio.2009.10.009.
Three thermotolerant "Absidia-like" isolates with unique morphologic characteristics, recovered from nasopharyngeal swab of a liver transplant recipient, gastric biopsy of a renal transplant recipient, and skin biopsy of a man with burn, respectively, were characterized. Microscopic examination showed nonseptate hyphae with highly branched sporangiophores. Uniquely, most side branches were circinate, and abundant pleomorphic giant cells with fingerlike projections were observed, characteristics absent from other Absidia/Lichtheimia spp. ITS1-5.8S-ITS2 rRNA gene cluster, partial EF1alpha gene, and partial beta-actin gene sequencing showed that the 3 strains formed a distinct cluster, most closely related to, but distinct from, Lichtheimia corymbifera, Lichtheimia blakesleeana, and Lichtheimia hyalospora. Based on the morphologic and genotypic characteristics, we propose a new species, Lichtheimia hongkongensis sp. nov., to describe this fungus, which caused rhinocerebral, gastrointestinal, and cutaneous mucormycosis, respectively, in 3 patients. A significant proportion of L. corymbifera associated with mucormycosis reported may be L. hongkongensis.
我们对分别从肝移植受者鼻拭子、肾移植受者胃活检和烧伤患者皮肤活检中分离到的 3 株形态独特的耐热“根霉样”分离株进行了鉴定。显微镜检查显示无隔菌丝和高度分枝的孢子梗。独特的是，大多数侧枝呈回旋状，并且观察到丰富的多形巨型细胞，具有指状突起，这是其他棘孢壳属/光壳霉属种所没有的特征。ITS1-5.8S-ITS2 rRNA 基因簇、部分 EF1alpha 基因和部分 β-actin 基因测序显示，这 3 株菌形成了一个独特的聚类，与棘孢壳属光壳霉、棘孢壳属布拉克尔斯利霉和棘孢壳属透明质霉最为密切相关，但又有区别。基于形态学和基因型特征，我们提出了一个新种，即香港光壳霉，以描述分别引起 3 名患者鼻脑、胃肠和皮肤毛霉病的这种真菌。与毛霉病相关的棘孢壳属光壳霉的比例很高，其中可能有一部分是香港光壳霉。
