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本文引用的文献

1
Low frequency nonnucleoside reverse-transcriptase inhibitor-resistant variants contribute to failure of efavirenz-containing regimens in treatment- experienced patients.低频非核苷类逆转录酶抑制剂耐药变异体导致接受含依非韦伦方案治疗的经验丰富患者治疗失败。
J Infect Dis. 2010 Mar;201(5):672-80. doi: 10.1086/650542.
2
Improvement in allele-specific PCR assay with the use of polymorphism-specific primers for the analysis of minor variant drug resistance in HIV-1 subtype C.使用多态性特异性引物改进等位基因特异性PCR检测法以分析HIV-1 C亚型中的次要变异耐药性。
J Virol Methods. 2008 Apr;149(1):69-75. doi: 10.1016/j.jviromet.2008.01.005. Epub 2008 Mar 4.
3
HIV-1 reverse transcriptase inhibitor resistance mutations and fitness: a view from the clinic and ex vivo.HIV-1逆转录酶抑制剂耐药性突变与适应性：临床及体外研究视角
Virus Res. 2008 Jun;134(1-2):104-23. doi: 10.1016/j.virusres.2007.12.021. Epub 2008 Mar 4.
4
Suppression of viremia and evolution of human immunodeficiency virus type 1 drug resistance in a macaque model for antiretroviral therapy.猕猴抗逆转录病毒治疗模型中1型人类免疫缺陷病毒病毒血症的抑制及耐药性演变
J Virol. 2007 Nov;81(22):12145-55. doi: 10.1128/JVI.01301-07. Epub 2007 Sep 12.
5
Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency.检测低频1型人类免疫缺陷病毒耐药突变体方法的盲法多中心比较。
J Clin Microbiol. 2006 Jul;44(7):2612-4. doi: 10.1128/JCM.00449-06.
6
Persistence of nevirapine-resistant HIV-1 in women after single-dose nevirapine therapy for prevention of maternal-to-fetal HIV-1 transmission.单剂量奈韦拉平治疗预防母婴HIV-1传播后，女性体内耐奈韦拉平HIV-1的持续存在情况。
Proc Natl Acad Sci U S A. 2006 May 2;103(18):7094-9. doi: 10.1073/pnas.0602033103. Epub 2006 Apr 25.
7
Decay of K103N mutants in cellular DNA and plasma RNA after single-dose nevirapine to reduce mother-to-child HIV transmission.单剂量奈韦拉平用于减少母婴HIV传播后K103N突变体在细胞DNA和血浆RNA中的衰减
AIDS. 2006 Apr 24;20(7):995-1002. doi: 10.1097/01.aids.0000222071.60620.1d.
8
Selection and persistence of non-nucleoside reverse transcriptase inhibitor-resistant HIV-1 in patients starting and stopping non-nucleoside therapy.开始和停止非核苷类治疗的患者中对非核苷类逆转录酶抑制剂耐药的HIV-1的选择与持续存在
AIDS. 2006 Mar 21;20(5):701-10. doi: 10.1097/01.aids.0000216370.69066.7f.
9
Emergence of drug-resistant HIV-1 after intrapartum administration of single-dose nevirapine is substantially underestimated.分娩期单剂量奈韦拉平给药后耐药性HIV-1的出现情况被大大低估了。
J Infect Dis. 2005 Jul 1;192(1):16-23. doi: 10.1086/430741. Epub 2005 Jun 1.
10
Rare mutations at codon 103 of HIV-1 reverse transcriptase can confer resistance to non-nucleoside reverse transcriptase inhibitors.人类免疫缺陷病毒1型逆转录酶第103位密码子的罕见突变可导致对非核苷类逆转录酶抑制剂产生耐药性。
AIDS. 2005 Mar 24;19(6):549-54. doi: 10.1097/01.aids.0000163930.68907.37.

利用患者特异性 HIV 共识序列进行引物设计的等位基因特异性 PCR 的优化。

Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.

机构信息

HIV Drug Resistance Program, NCI, NIH, Frederick, MD, United States.

出版信息

J Virol Methods. 2010 Mar;164(1-2):122-6. doi: 10.1016/j.jviromet.2009.11.025. Epub 2009 Dec 3.

DOI:10.1016/j.jviromet.2009.11.025
PMID:19948190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4734888/
Abstract

Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance.

摘要

基于亚型共有序列的等位基因特异性 PCR 是一种检测 HIV-1 感染患者中低频耐药突变体的有效技术。然而，这种方法可能会受到与引物互补区域遗传变异的限制，从而导致等位基因检测的可变性。本研究的目的是量化这种影响，然后提高检测性能。