Serviço de Virologia do Instituto Adolfo Lutz, São Paulo, SP, Brazil.
J Med Virol. 2010 Jan;82(1):175-85. doi: 10.1002/jmv.21606.
Nucleotide sequences of two regions of the genomes of 11 yellow fever virus (YFV) samples isolated from monkeys or humans with symptomatic yellow fever (YF) in Brazil in 2000, 2004, and 2008 were determined with the objective of establishing the genotypes and studying the genetic variation. Results of the Bayesian phylogenetic analysis showed that sequences generated from strains from 2004 and 2008 formed a new subclade within the clade 1 of the South American genotype I. The new subgroup is here designated as 1E. Sequences of YFV strains recovered in 2000 belong to the subclade 1D, which comprises previously characterized YFV strains from Brazil. Molecular dating analyses suggested that the new subclade 1E started diversifying from 1D about 1975 and that the most recent 2004-2008 isolates arose about 1985.
对 2000 年、2004 年和 2008 年巴西因出现黄热病（YF）症状而从猴子或人类中分离出的 11 株黄热病毒（YFV）样本的基因组两个区域的核苷酸序列进行了测定，目的是确定基因型并研究遗传变异。贝叶斯系统发育分析结果表明，2004 年和 2008 年分离株产生的序列在南美洲基因型 I 的第 1 分支内形成了一个新的亚分支。新的亚群在此被指定为 1E。2000 年分离的 YFV 株序列属于 1D 亚分支，该亚分支包含先前从巴西分离的特征明确的 YFV 株。分子年代分析表明，新的 1E 亚分支于 1975 年左右从 1D 分支开始多样化，而最近的 2004-2008 年分离株大约在 1985 年出现。
