de Filippis A M B, Nogueira R M R, Schatzmayr H G, Tavares D S, Jabor A V, Diniz S C M, Oliveira J C, Moreira E, Miagostovich M P, Costa E V, Galler R
Laboratório de Flavivírus, Departamento de Virologia, Instituto Oswaldo Cruz, Fiocruz, RJ, Brasil.
J Med Virol. 2002 Dec;68(4):620-7. doi: 10.1002/jmv.10226.
Between January and March 2001, an outbreak of jaundice and hemorrhagic fever occurred in the state of Minas Gerais, Southeast region of Brazil, in which a mortality rate of 53% was reported. Seroconversion, virus isolation, histopathological and immunohistochemical findings, and reverse transcription-polymerase chain reaction (RT-PCR) identified yellow fever virus (YFV) as the etiological agent responsible for the outbreak. Partial nucleotide sequence analysis from a fragment of the YFV genome spanning parts of nonstructural (NS) 5 gene and 3' noncoding region (3' UTR) showed that the YFV involved in this outbreak belongs to South American genotype I and differs from the Brazilian virus identified in 1996.
2001年1月至3月期间，巴西东南部米纳斯吉拉斯州爆发了黄疸和出血热疫情，据报告死亡率为53%。血清学转换、病毒分离、组织病理学和免疫组化检查结果以及逆转录聚合酶链反应（RT-PCR）确定黄热病毒（YFV）是此次疫情的病原体。对YFV基因组一个片段进行的部分核苷酸序列分析，该片段跨越非结构（NS）5基因部分和3'非编码区（3'UTR），结果显示此次疫情涉及的YFV属于南美基因型I，与1996年鉴定的巴西病毒不同。
