Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742, USA.
J Agric Food Chem. 2009 Dec 9;57(23):11174-85. doi: 10.1021/jf902609n.
This study characterized and compared 18 colored seed coat soybeans for the isoflavone, total phenolic, and cyanidin-3-glucoside (Cy-3-glc) contents of their flour extracts and the fatty acid composition and carotenoid and alpha-tocopherol contents of their oils. Antioxidant assays also assessed activity of the flour extracts against peroxyl, hydroxyl, and ABTS(+) radicals. Black seed coat soybeans had the highest TPC, ORAC, HOSC, and ABTS(+) radical scavenging values, in addition to the highest isoflavone content, and were the only color to contain Cy-3-glc. Five soybeans (two black and one each brown, yellow, and green) were selected to test their effects on HT-29 human colorectal cancer cell growth. The effects of the hydrolyzed and unhydrolyzed extracts were compared to an aglycone isoflavone standard mixture of the same total molar concentration as the highest soybean concentration of 15 mg of seed flour equiv/mL of treatment media. All high doses of hydrolyzed soybean treatments except the green genotype significantly reduced cell number compared to control at 3 h of treatment time, whereas the high dose of isoflavone standard treatment took 72 h to show a significant reduction (P < 0.05).
本研究对 18 种有色种皮大豆的面粉提取物中的异黄酮、总酚和矢车菊素-3-葡萄糖苷（Cy-3-glc）含量、油中的脂肪酸组成、类胡萝卜素和α-生育酚含量进行了表征和比较。抗氧化测定还评估了面粉提取物对过氧自由基、羟自由基和 ABTS(+)自由基的活性。黑色种皮大豆的 TPC、ORAC、HOSC 和 ABTS(+)自由基清除值最高，异黄酮含量最高，也是唯一含有 Cy-3-glc 的颜色。选择了五种大豆（两种黑色和一种棕色、黄色和绿色）来测试它们对 HT-29 人结肠癌细胞生长的影响。将水解和未水解提取物的作用与相同总摩尔浓度的糖苷异黄酮标准混合物（最高大豆浓度为 15mg 种子粉当量/mL 处理介质）进行比较。与对照相比，除了绿色基因型外，所有高剂量水解大豆处理在 3 小时的处理时间内均显著降低了细胞数量，而高剂量异黄酮标准处理则需要 72 小时才能显示出显著降低（P<0.05）。
