Flores Paulina Calderon, Yoon Jin Seok, Kim Dae Yeon, Seo Yong Weon
Department of Plant Biotechnology, Korea University, Seoul, Korea.
Ojeong Plant Breeding Research Center, Korea University, Seoul, Korea.
Evol Bioinform Online. 2022 Apr 13;18:11769343221093341. doi: 10.1177/11769343221093341. eCollection 2022.
Plants accumulate key metabolites as a response of biotic/abiotic stress conditions. In seed coats, anthocyanins, carotenoids, and chlorophylls can be found. They have been associated as important antioxidants that affect germination. In wheat, anthocyanins can impart the seed coat color which have been recognized as health-promoting nutrients. Transcription factors act as master regulators of cellular processes. Transcription complexes such as MYB-bHLH-WD40 (MBW) regulate the expression of multiple target genes in various plant species. In this study, the spatiotemporal accumulation of seed coat pigments in different developmental stages (10, 20, 30, and 40 days after pollination) was analyzed using cryo-cuts. Moreover, the accumulation of phenolic, anthocyanin, and chlorophyll contents was quantified, and the expression of flavonoid biosynthetic genes was evaluated. Finally, transcriptome analysis was performed to analyze putative MYB genes related to seed coat color, followed by further characterization of putative genes. , an MYB gene, was cloned and sequenced. It was determined that contains a SANT domain, which is often present in proteins participating in the response to anthocyanin accumulation. Moreover, transcript levels were shown to be influenced by anthocyanin accumulation during grain development. Interaction network analysis showed interactions with GL2 (HD-ZIP IV), EGL3 (bHLH), and TTG1 (WD40). The findings of this study elucidate the mechanisms underlying color formation in L. seed coats.
植物积累关键代谢产物以应对生物/非生物胁迫条件。在种皮中，可以发现花青素、类胡萝卜素和叶绿素。它们被认为是影响种子萌发的重要抗氧化剂。在小麦中，花青素可赋予种皮颜色，而种皮颜色已被公认为具有促进健康的营养成分。转录因子作为细胞过程的主要调节因子。转录复合体如MYB-bHLH-WD40（MBW）在多种植物物种中调节多个靶基因的表达。在本研究中，使用冷冻切片分析了不同发育阶段（授粉后10、20、30和40天）种皮色素的时空积累。此外，对酚类、花青素和叶绿素含量的积累进行了定量，并评估了类黄酮生物合成基因的表达。最后，进行转录组分析以分析与种皮颜色相关的假定MYB基因，随后对假定基因进行进一步表征。克隆并测序了一个MYB基因。确定该基因含有一个SANT结构域，该结构域通常存在于参与花青素积累响应的蛋白质中。此外，该基因的转录水平在籽粒发育过程中受花青素积累的影响。相互作用网络分析表明该基因与GL2（HD-ZIP IV）、EGL3（bHLH）和TTG1（WD40）存在相互作用。本研究结果阐明了L.种皮颜色形成的潜在机制。
