Institute for Advanced Chemistry of Catalonia, CSIC (ICAQ-CSIC), Barcelona, Spain.
J Agric Food Chem. 2009 Dec 9;57(23):11418-26. doi: 10.1021/jf901972c.
Grape antioxidant dietary fiber (GADF) combines the putative health benefits of fiber and polyphenols. Polyphenolic metabolites may play a key role in the overall biological effects of this supplement. We identified phenolic GADF metabolites in rat urine at different times after oral administration, using HPLC-ESI-MS/MS techniques. The phenolic metabolic outcome of GADF is essentially an array of mono- and polyconjugated epicatechins and free or conjugated smaller phenolic acids, some of them never reported before. We have detected 18 mono-, di-, and triconjugates of epicatechin with glucuronide, methyl and sulfate moieties and small phenolic acids both free and conjugated. The results suggest that the procyanidin oligomers are both depolymerized in the digestive tract into epicatechin conjugates and degraded by the colonic microbiota into phenolic acids and their conjugates. For several hours after ingestion of GADF, a great variety of phenolic species, including some with an intact catechol group, are in contact with the digestive tract tissues before, during and after metabolization, and many of them are systemically bioavailable before being excreted.
葡萄抗氧化膳食纤维 (GADF) 结合了纤维和多酚的潜在健康益处。多酚代谢物可能在这种补充剂的整体生物学效应中起关键作用。我们使用 HPLC-ESI-MS/MS 技术在大鼠口服后不同时间的尿液中鉴定了酚类 GADF 代谢物。GADF 的酚类代谢产物本质上是一系列单和多共轭表儿茶素以及游离或共轭的较小酚酸，其中一些以前从未报道过。我们已经检测到 18 种表儿茶素的单、二和三共轭物，具有葡萄糖醛酸苷、甲基和硫酸盐部分以及游离和共轭的小酚酸。结果表明，原花青素低聚物在消化道中既解聚成表儿茶素缀合物，又被结肠微生物群降解成酚酸及其缀合物。在摄入 GADF 后的几个小时内，在代谢前、中、后，各种酚类物质，包括一些具有完整儿茶酚基团的物质，与消化道组织接触，其中许多在排泄前具有系统生物利用度。
