Institute of Natural Products Chemistry, VAST.
Biol Pharm Bull. 2009 Dec;32(12):1952-6. doi: 10.1248/bpb.32.1952.
Nine compounds, including six phenylethanoid glycosides: acteoside (1); bioside (2); echinacoside (3); poliumoside (4); phenylethyl glycoside (5); salidroside (6) and three flavonoids; linarin (7); apigenin (8); isorhoifolin (9), were isolated from the flowers of Buddleja officinalis MAXIM. (Buddlejaceae). Chemical structures were confirmed by (1)H-, and (13)C-NMR, and MS spectral methods and compared with those reported in the literature. Antioxidant activities of the methanol and water extracts, and all isolated compounds were evaluated using the total oxidant scavenging capacity (TOSC) assay against peroxynitrite. Results of the assay showed that the phenylethanoid glycosides, a major class of compounds of the flowers of B. officinalis, possess strong antioxidant activity. Of these, acteoside, echinacoside and poliumoside have 9.9-, 9.8- and 9.5-fold TOSC value, respectively, compared with the positive control, Trolox.
从黄花鹤顶兰（Orchidaceae）中分离得到 9 种化合物，包括 6 种苯乙醇苷类化合物：毛蕊花糖苷（1）；梓醇苷（2）；麦角甾苷（3）；蒲公英赛醇苷（4）；苯乙基糖苷（5）；红景天苷（6）和 3 种黄酮类化合物：圣草酚-7-O-葡萄糖苷（7）；芹菜素（8）；异荭草苷（9）。通过（1）H-NMR、（13）C-NMR 和 MS 光谱方法确定了它们的化学结构，并与文献报道的结构进行了比较。采用对过氧亚硝酸盐的总抗氧化能力（TOSC）测定法，评估了甲醇和水提取物以及所有分离得到的化合物的抗氧化活性。测定结果表明，苯乙醇苷类化合物是黄花鹤顶兰的主要类化合物，具有很强的抗氧化活性。其中，毛蕊花糖苷、麦角甾苷和蒲公英赛醇苷的 TOSC 值分别是阳性对照 Trolox 的 9.9 倍、9.8 倍和 9.5 倍。
