Zhang Min, Miao Yujing, Zhang Xinke, Sun Xiao, Li Minhui, Huang Linfang
Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, College of Pharmacy, Baotou Medical College, Baotou, China.
Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Front Microbiol. 2023 Jun 27;14:1154688. doi: 10.3389/fmicb.2023.1154688. eCollection 2023.
Plant microorganism is critical to plant health, adaptability, and productive forces. Intriguingly, the metabolites and microorganisms can act upon each other in a plant. The union of metabolomics and microbiome may uncover the crucial connections of the plant to its microbiome. It has important benefits for the agricultural industry and human being health, particularly for Chinese medical science investigation.
In this last 2 years study, on the strength of the UPLC-MS/MS detection platform, we accurately qualitatively, and quantitatively measured the fleshy stems of two ecotypes. Thereafter, through high-throughput amplicon sequencing 16S/ITS sequences were procured.
PhGs metabolites including echinacoside, isoacteoside, and cistanoside A were significantly downregulated at two ecotypes of . Add up to 876 metabolites were monitored and 231 differential metabolites were analyzed. Further analysis of 34 core differential metabolites showed that 15 compounds with up-regulated belonged to phenolic acids, flavonoids, and organic acids, while 19 compounds with down-regulated belonged to phenolic acids, flavonoids, alkaloids, amino acids, lipids, and nucleotides. There was no noteworthy discrepancy in the endophytic bacteria's and diversity between sandy and loam ecotypes. By comparison, the and diversity of endophytic fungi was notably distinct. The fungal community of the loam ecotype is more abundant than the sandy ecotype. However, there were few such differences in bacteria. Most abundant genera included typical endophytes such as , , , and . LEfSe results revealed there were 11 and 20 biomarkers of endophytic bacteria and fungi in at two ecotypes, respectively. The combination parsing of microflora and metabolites indicated noteworthy relativity between the endophytic fungal communities and metabolite output. Key correlation results that was positive relation with Syringin, is negative relation with 7-methylxanthine and is completely positively correlated with nepetin-7--alloside.
The aim of this research is: (1) to explore firstly the influence of ecotype on from the perspective of endophytes and metabolites; (2) to investigate the relationship between endophytes and metabolites. This discovery advances our understanding of the interaction between endophytes and plants and provides a theoretical basis for cultivation of in future.
植物微生物对植物健康、适应性和生产力至关重要。有趣的是，代谢物和微生物在植物中会相互作用。代谢组学和微生物组的结合可能揭示植物与其微生物组之间的关键联系。这对农业产业和人类健康具有重要益处，尤其对于中医药科学研究。
在过去两年的研究中，基于超高效液相色谱-串联质谱检测平台，我们准确地对两种生态型的肉质茎进行了定性和定量测量。此后，通过高通量扩增子测序获得了16S/ITS序列。
包括松果菊苷、异毛蕊花糖苷和肉苁蓉苷A在内的苯乙醇苷类代谢物在两种生态型中均显著下调。共监测到876种代谢物，并分析了231种差异代谢物。对34种核心差异代谢物的进一步分析表明，上调的15种化合物属于酚酸、黄酮类和有机酸，而下调的19种化合物属于酚酸、黄酮类、生物碱、氨基酸、脂质和核苷酸。沙质和壤土生态型内生细菌的丰富度和多样性没有显著差异。相比之下，内生真菌的丰富度和多样性明显不同。壤土生态型的真菌群落比沙质生态型更丰富。然而，细菌方面几乎没有此类差异。最丰富的属包括典型的内生菌，如[具体属名未给出]、[具体属名未给出]、[具体属名未给出]、[具体属名未给出]和[具体属名未给出]。线性判别分析效应大小（LEfSe）结果显示，在两种生态型的[具体名称未给出]中，内生细菌和真菌分别有11个和20个生物标志物。微生物群和代谢物的联合解析表明内生真菌群落与代谢物产出之间存在显著相关性。关键相关结果表明，[具体菌名未给出]与紫丁香苷呈正相关，[具体菌名未给出]与7-甲基黄嘌呤呈负相关，[具体菌名未给出]与荆芥苷-7-O-葡萄糖苷完全正相关。
本研究的目的是：（1）首先从内生菌和代谢物的角度探索生态型对[具体名称未给出]的影响；（2）研究内生菌与代谢物之间的关系。这一发现增进了我们对内生菌与植物之间相互作用的理解，并为未来[具体名称未给出]的栽培提供了理论依据。
