Department of Resources Science of Traditional Chinese Medicine, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, P. R. China.
The Sixth People's Hospital of Hengshui, Hengshui, P. R. China.
J Sep Sci. 2020 Apr;43(8):1450-1457. doi: 10.1002/jssc.201901062. Epub 2020 Feb 26.
Buddleja officinalis Maxim., a deciduous, flowering shrub, is used as a traditional Chinese medicine; the bioactivity of B. officinalis is primarily due to flavonoids and phenylethanoid glycosides. In the study, acteoside and linarin were successfully isolated from B. officinalis by high-speed countercurrent chromatography with a two-phase solvent system composed of ethyl acetate: n-butanol: water (5:0.8:5, v/v/v). The purities of acteoside and linarin were determined to be 97.3 and 98.2%, respectively, using one-step high-speed countercurrent chromatography separation. The chemical structures of the two compounds were identified by electrospray ionization-mass spectrometry and nuclear magnetic resonance. After separation, the anti-inflammatory effects of the two compounds were evaluated using lipopolysaccharide-induced human umbilical vein endothelial cells. Acteoside and linarin inhibited the expression of nitric oxide, tumor necrosis factor α and interleukin 1β, which demonstrated that acteoside and linarin possessed anti-inflammatory activity.
黄花丹，一种落叶开花灌木，被用作中药；黄花丹的生物活性主要归因于类黄酮和苯乙醇苷。在这项研究中，黄花丹中的紫茎泽兰苷和圣草酚通过两相溶剂系统（乙酸乙酯：正丁醇：水（5：0.8：5，v/v/v）组成的高速逆流色谱法成功分离。采用一步高速逆流色谱分离法，紫茎泽兰苷和圣草酚的纯度分别确定为 97.3%和 98.2%。通过电喷雾电离-质谱和核磁共振鉴定了这两种化合物的化学结构。分离后，采用脂多糖诱导的人脐静脉内皮细胞评价了两种化合物的抗炎作用。紫茎泽兰苷和圣草酚抑制了一氧化氮、肿瘤坏死因子 α 和白细胞介素 1β 的表达，表明紫茎泽兰苷和圣草酚具有抗炎活性。
