Delazar Abbas, Sabzevari Araz, Mojarrab Mahdi, Nazemiyeh Hossein, Esnaashari Solmaz, Nahar Lutfun, Razavi Seied Mehdi, Sarker Satyajit Dey
School of Pharmacy and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Nat Med. 2008 Oct;62(4):464-6. doi: 10.1007/s11418-008-0255-y. Epub 2008 May 17.
Seven free-radical-scavenging phenolic compounds including five flavonoids, rutin (1), chrysoeriol 7-O-rutinoside (2), kaempferol 3-O-glucoside (3), chrysoeriol 7-O-glucoside (4) and naringenin (5), and two phenylethanoid glycosides, forsythoside B (6) and acteoside (7) were isolated from the methanol extract of the aerial parts of the Iranian medicinal plant Phlomis caucasica by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated by spectroscopic means. The free-radical-scavenging properties of 1-7 were assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay. Among these compounds, forsythoside B (6) and acteoside (7) were found to be the most potent antioxidants with the RC(50) values of 4.97 and 4.27 microg/ml, respectively.
通过反相制备高效液相色谱法（HPLC）从伊朗药用植物高加索糙苏地上部分的甲醇提取物中分离出七种自由基清除酚类化合物，包括五种黄酮类化合物，芦丁（1）、 Chrysoeriol 7-O-芸香糖苷（2）、山奈酚3-O-葡萄糖苷（3）、 Chrysoeriol 7-O-葡萄糖苷（4）和柚皮素（5），以及两种苯乙醇苷，连翘酯苷B（6）和毛蕊花糖苷（7），并通过光谱手段阐明了这些化合物的结构。通过2,2-二苯基-1-苦基肼基（DPPH）测定法评估了1-7的自由基清除特性。在这些化合物中，连翘酯苷B（6）和毛蕊花糖苷（7）被发现是最有效的抗氧化剂，其RC(50)值分别为4.97和4.27微克/毫升。
