Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), AC Nieuwesluis, The Netherlands.
Microb Ecol. 2010 Jan;59(1):109-20. doi: 10.1007/s00248-009-9614-9.
We studied the response of the sulfate-reducing prokaryote (SRP) communities to the experimental variation of salinity and tide in an outdoor mesocosm setup. Intact soil monoliths were collected at two areas of the Haringvliet lagoon (The Netherlands): one sampling location consisted of agricultural grassland, drained and fertilized for at least the last century; the other of a freshwater marshland with more recent sea influence. Two factors, i.e., "salinity" (freshwater/oligohaline) and "tide" (nontidal/tidal), were tested in a full-factorial design. Soil samples were collected after 5 months (June-October). Dissimilatory (bi)sulfite reductase beta subunit-based denaturing gradient gel electrophoresis (dsrB-DGGE) analysis revealed that the SRP community composition in the agricultural grassland and in the freshwater marshland was represented mainly by microorganisms related to the Desulfobulbaceae and the Desulfobacteraceae, respectively. Desulfovibrio-related dsrB were detected only in the tidal treatments; Desulfomonile-related dsrB occurrence was related to the presence of oligohaline conditions. Treatments did have an effect on the overall SRP community composition of both soils, but not on the sulfate depletion rates in sulfate-amended anoxic slurry incubations. However, initiation of sulfate reduction upon sulfate addition was clearly different between the two soils.
我们研究了硫酸盐还原原核生物（SRP）群落对盐度和潮汐实验变化的响应，这是在户外中观模型装置中进行的。从哈林维利特泻湖（荷兰）的两个区域采集了完整的土壤单体：一个采样点由农业草地组成，至少在上个世纪就已经排干和施肥；另一个采样点是淡水沼泽地，最近受到了海水的影响。在完全因子设计中测试了两个因素，即“盐度”（淡水/寡盐）和“潮汐”（非潮汐/潮汐）。土壤样品在 5 个月后采集（6 月至 10 月）。基于异化（双）亚硫酸盐还原酶β亚基的变性梯度凝胶电泳（dsrB-DGGE）分析表明，农业草地和淡水沼泽地的 SRP 群落组成主要由与脱硫杆菌科和脱硫杆菌科相关的微生物代表。只有在潮汐处理中检测到与脱硫弧菌相关的 dsrB；Desulfomonile 相关的 dsrB 发生与寡盐条件的存在有关。处理确实对两种土壤的总体 SRP 群落组成有影响，但对硫酸盐添加的缺氧泥浆培养中的硫酸盐消耗率没有影响。然而，硫酸盐添加后硫酸盐还原的起始在两种土壤之间明显不同。
