Division of Rheumatology, Department of Internal Medicine, School of Medicine, Pusan National University, 1-10, Ami-Dong, Seo-Gu, Busan, South Korea.
Clin Rheumatol. 2010 Mar;29(3):273-9. doi: 10.1007/s10067-009-1316-7. Epub 2009 Dec 2.
The aim of this study was to investigate the expressions of Toll-like receptor (TLR) 2, TLR4, TLR9, and their correlations with the expression of cytokines that are associated with activation of CD4(+) T cells and inflammation including interferon gamma (IFNgamma), interleukin 4 (IL4), interleukin 17 (IL17), and tumor necrosis factor alpha (TNFalpha) in muscle tissues of patients with dermatomyositis (DM) and polymyositis (PM). The expressions of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were measured by real-time reverse transcription-polymerase chain reaction in muscle tissues from 14 patients with DM and PM (nine patients with DM, five patients with PM) and three controls. The expressions of TLR2, TLR4, and TLR9 were also localized with immunohistochemistry. The expression levels of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were significantly high in patients with DM and PM compared with those in the controls, and the expression levels of TLR4 and TLR9 had significant positive correlations with the expressions of IFNgamma, IL4, IL17, and TNFalpha. Immunohistochemistry showed that TLR2, TLR4, and TLR9 were expressed by infiltrating cells of perimysium in DM, whereas they were expressed by infiltrating cells of endomysium in PM. These results suggest that the involvement of TLR4 and TLR9 in immunopathogenesis of DM and PM might be connected with activation of CD4(+) T cells.
本研究旨在探讨 Toll 样受体（TLR）2、TLR4、TLR9 的表达及其与与 CD4+T 细胞激活和炎症相关细胞因子（包括干扰素 γ（IFNγ）、白细胞介素 4（IL4）、白细胞介素 17（IL17）和肿瘤坏死因子 α（TNFα））的表达之间的相关性，这些细胞因子存在于皮肌炎（DM）和多发性肌炎（PM）患者的肌肉组织中。通过实时逆转录-聚合酶链反应（real-time RT-PCR），在 14 例 DM 和 PM 患者（9 例 DM，5 例 PM）和 3 例对照者的肌肉组织中检测 TLR2、TLR4、TLR9、IFNγ、IL4、IL17 和 TNFα 的表达。用免疫组织化学法定位 TLR2、TLR4 和 TLR9 的表达。与对照组相比，DM 和 PM 患者的 TLR2、TLR4、TLR9、IFNγ、IL4、IL17 和 TNFα 的表达水平显著升高，TLR4 和 TLR9 的表达水平与 IFNγ、IL4、IL17 和 TNFα 的表达水平呈显著正相关。免疫组织化学显示，TLR2、TLR4 和 TLR9 在 DM 的肌束膜浸润细胞中表达，而在 PM 的肌内膜浸润细胞中表达。这些结果提示 TLR4 和 TLR9 可能参与 DM 和 PM 的免疫发病机制，与 CD4+T 细胞的激活有关。
