Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570 020, India.
Biotechnol Lett. 2010 Apr;32(4):503-6. doi: 10.1007/s10529-009-0176-1. Epub 2009 Dec 2.
Vitamin B(12) was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B(12)/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B(12) for analysis. The presence of the K(+) adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization-mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.
在分批培养（96 h）中，益生菌植物乳杆菌在厌氧和好氧阶段各持续 48 h 来生产维生素 B(12)，产量为 13 ng 维生素 B(12)/g 干生物量。用氰化钠介导的细胞裂解，然后用苯甲醇/氯仿/水提取，提高了细胞内维生素 B(12)的释放量以供分析。利用电喷雾电离质谱确定了氰钴胺素（m/z 为 1394）的 K(+)加合物的存在；在存在氰钴胺素的情况下培养大肠杆菌突变体，确定了其生物利用度。
