Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
World J Microbiol Biotechnol. 2013 Sep;29(9):1713-22. doi: 10.1007/s11274-013-1334-0. Epub 2013 Mar 30.
Aerobic metabolism and response to oxidative stress and starvation were studied in 11 Lactobacillus plantarum, L. paraplantarum and L. pentosus strains in order to assess the impact of aerobic metabolism on the growth and on the stress response. The strains were grown in aerobiosis without supplementation (AE), with hemin (AEH) or with hemin and menaquinone (AEHM) supplementation and in anaerobiosis (AN) in a complex buffered substrate. Growth rate, biomass yield, glucose and O₂ consumption, production of lactic acid and H₂O₂, catalase activity, oxidative and starvation stress tolerance were evaluated. Aerobic growth increased biomass yield in late stationary phase. Further increase in yield was obtained with both hemin (H) and menaquinone (M) addition. With few exceptions, the increase in biomass correlated with the decrease of lactic acid which, however, decreased in anaerobic conditions as well in some strains. Addition of H or H + M increased growth rate for some strains but reduced the duration of the lag phase. H₂O₂ production was found only for aerobic growth with no supplementation due to catalase production when hemin was supplemented. To our knowledge this is the first study in which the advantages of aerobic growth with H or H + M in improving tolerance of oxidative stress and long-term survival is demonstrated on several strains of the L. plantarum group. The results may have significant technological consequences for both starter and probiotic production.
为了评估需氧代谢对生长和应激反应的影响，研究了 11 株植物乳杆菌、副干酪乳杆菌和戊糖片球菌的需氧代谢和对氧化应激及饥饿的反应。在有氧条件下（AE）、添加血红素（AEH）或血红素和甲萘醌（AEHM）的条件下以及在复杂缓冲底物的厌氧条件下（AN）培养菌株。评估了生长速率、生物量产量、葡萄糖和 O₂消耗、乳酸和 H₂O₂的产生、过氧化氢酶活性、氧化应激和饥饿应激耐受性。好氧生长增加了晚期停滞期的生物量产量。添加血红素（H）和甲萘醌（M）进一步增加了产量。除了少数例外，生物量的增加与乳酸的减少相关，但在某些菌株的厌氧条件下，乳酸也会减少。添加 H 或 H + M 可提高一些菌株的生长速率，但会缩短迟滞期。由于添加血红素时会产生过氧化氢酶，因此仅在有氧生长且未添加任何物质的情况下才会产生 H₂O₂。据我们所知，这是首次在植物乳杆菌属的多个菌株上研究有氧生长添加 H 或 H + M 以提高氧化应激和长期存活能力的优势。这些结果可能对启动子和益生菌生产都具有重要的技术意义。
