Liu Qiuling, Lu Dejian, Zhao Hu
Faculty of Forensic Medicine, Sun Yat-sen University Zhongshan School of Medicine, Guangzhou, Guangdong, 510080 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Dec;26(6):664-9. doi: 10.3760/cma.j.issn.1003-9406.2009.06.012.
To establish a nine X-chromosome short tandem repeats (X-STR) loci multiplex PCR method and study the polymorphism of the 9 X-STR loci,and to determine its application in kinship tests for forensic medicine.
A fluorescent multiplex PCR that simultaneously amplifies 9 X-STR loci, i.e. DXS7133, DXS981, DXS7424, DXS6789, DXS7132, GATA165B12, DXS101, GATA31E08 and DXS10011, were set up. PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer with GeneMapper ID 3.1 analysis software.
When 251 unrelated male and 112 unrelated female individuals from southern China were tested, 111 alleles were detected. The power of discrimination in females was 0.5837-0.9959. Mean exclusion chance for X-STR in standard trios with daughters was 0.4072-0.9511. Polymorphism information content was 0.4481-0.9531.
The results demonstrate that the 9 loci in the multiplex system provide high polymorphism information, and the multiplex system provides a fast technology for forensic identification and paternity testing. The X-STR multiplex system can complement the analysis of AS-STR and Y-STR efficiently.
建立一种9个X染色体短串联重复序列（X-STR）位点的多重聚合酶链反应（PCR）方法，研究这9个X-STR位点的多态性，并确定其在法医学亲缘关系鉴定中的应用。
建立一种荧光多重PCR方法，可同时扩增9个X-STR位点，即DXS7133、DXS981、DXS7424、DXS6789、DXS7132、GATA165B12、DXS101、GATA31E08和DXS10011。PCR产物采用毛细管电泳和ABI prism 3100遗传分析仪，结合GeneMapper ID 3.1分析软件进行分析。
对来自中国南方的251名无关男性个体和112名无关女性个体进行检测，共检测到111个等位基因。女性个体的个体识别率为0.5837 - 0.9959。在有女儿的标准三联体中，X-STR的平均排除率为0.4072 - 0.9511。多态信息含量为0.4481 - 0.9531。
结果表明，该多重体系中的9个位点提供了高多态性信息，该多重体系为法医学鉴定和亲子鉴定提供了一种快速技术。X-STR多重体系能够有效地补充常染色体STR（AS-STR）和Y染色体STR（Y-STR）的分析。
