Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, 74, Zhongshan 2nd Road, Guangzhou 510080, People's Republic of China.
Mol Biol Rep. 2011 Nov;38(8):4879-83. doi: 10.1007/s11033-010-0629-6. Epub 2010 Dec 14.
This study is to explore the polymorphic nature of X-Chromosome short tandem repeats (ChrX STRs) loci, and to determine its application in kinship tests for forensic cases. A new fluorescent multiplex PCR that simultaneously amplifies the 10 ChX STRs loci in the same PCR reaction had been set up. DXS7132, DXS981, DXS6801, DXS6809, DXS6789, DXS7424, DXS101, DXS7133, GATA165B12 and GATA31E08 were analyzed in a sample of 511 (399 males and 112 females) unrelated individuals from Guangdong Han nationality in China. One hundred and one alleles were observed in all the loci. Here, we investigated the allele frequencies and mutation rates of the ten loci, and then made the comparison of allele frequencies distribution among different populations. The results show the ten loci in the multiplex systems may provide high polymorphism information for kinship testing and relationship investigations, and it is necessary to gain allele frequency and mutation rate of different population for forensic application.
本研究旨在探索 X 染色体短串联重复（ChrX STR）位点的多态性，并确定其在法医亲缘关系鉴定中的应用。本研究建立了一种新的荧光多重 PCR 方法，可在同一 PCR 反应中同时扩增 10 个 ChrX STR 位点。对来自中国广东汉族的 511 名（399 名男性和 112 名女性）无关个体样本进行了 DXS7132、DXS981、DXS6801、DXS6809、DXS6789、DXS7424、DXS101、DXS7133、GATA165B12 和 GATA31E08 10 个位点的分析。在所有位点共观察到 101 个等位基因。本研究调查了这 10 个位点的等位基因频率和突变率，并比较了不同人群的等位基因频率分布。结果表明，该多重体系中的 10 个位点可为亲缘关系鉴定和关系调查提供丰富的多态性信息，有必要获得不同人群的等位基因频率和突变率，以用于法医应用。
