Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA.
ACS Nano. 2010 Jan 26;4(1):35-42. doi: 10.1021/nn901392m.
Gold nanocages with localized surface plasmon resonance peaks in the near-infrared region exhibited a broad two-photon photoluminescence band extending from 450 to 650 nm when excited by a Ti:sapphire laser at 800 nm. The bright luminescence makes it possible to explore the use of Au nanocages as a new class of optical imaging agents for two-photon microscopy. In this work, we have demonstrated the use of two-photon microscopy as a convenient tool to directly examine the uptake of antibody-conjugated and PEGylated Au nanocages by U87MGwtEGFR cells. We have also correlated the results from two-photon microscopy with the data obtained by inductively coupled plasma mass spectrometry. Combined together, these results indicate that the antibody-conjugated Au nanocages were attached to the surface of the cells through antibody-antigen binding and then internalized into the cells via receptor-mediated endocytosis. The cellular uptake process was dependent on a number of parameters, including incubation time, incubation temperature, size of the Au nanocages, and the number of antibodies immobilized on each nanocage.
当用钛宝石激光在 800nm 激发时，具有局域表面等离子体共振峰在近红外区域的金纳米笼表现出从 450nm 到 650nm 的宽双光子光致发光带。明亮的发光使得有可能探索使用 Au 纳米笼作为双光子显微镜的新一类光学成像剂。在这项工作中，我们已经证明了使用双光子显微镜作为一种方便的工具，直接检查抗体偶联和聚乙二醇化 Au 纳米笼被 U87MGwtEGFR 细胞摄取。我们还将双光子显微镜的结果与电感耦合等离子体质谱获得的数据相关联。综合这些结果表明，抗体偶联的 Au 纳米笼通过抗体-抗原结合附着在细胞表面上，然后通过受体介导的内吞作用进入细胞内。细胞摄取过程取决于许多参数，包括孵育时间、孵育温度、Au 纳米笼的大小和固定在每个纳米笼上的抗体数量。
