褐藻岩藻黄醇通过抗氧化活性和抑制特定细胞色素 P450 酶对体外化学预防的潜力。

In vitro chemopreventive potential of fucophlorethols from the brown alga Fucus vesiculosus L. by anti-oxidant activity and inhibition of selected cytochrome P450 enzymes.

机构信息

Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, D-53115 Bonn, Germany.

出版信息

Phytochemistry. 2010 Feb;71(2-3):221-9. doi: 10.1016/j.phytochem.2009.10.020. Epub 2009 Dec 1.

DOI:10.1016/j.phytochem.2009.10.020

PMID:19954804

Abstract

Within a project focusing on the chemopreventive potential of algal phenols, two phloroglucinol derivatives, belonging to the class of fucophlorethols, and the known fucotriphlorethol A were obtained from the ethanolic extract of the brown alga Fucus vesiculosus L. The compounds trifucodiphlorethol A and trifucotriphlorethol A are composed of six and seven units of phloroglucinol, respectively. The compounds were examined for their cancer chemopreventive potential, in comparison with the monomer phloroglucinol. Trifucodiphlorethol A, trifucotriphlorethol A as well as fucotriphlorethol A were identified as strong radical scavengers, with IC(50) values for scavenging of 1,1-diphenyl-2 picrylhydrazyl radicals (DPPH) in the range of 10.0-14.4 microg/ml. All three compounds potently scavenged peroxyl radicals in the oxygen radical absorbance capacity (ORAC) assay. In addition, the compounds were shown to inhibit cytochrome P450 1A activity with IC(50) values in the range of 17.9-33 microg/ml, and aromatase (Cyp19) activity with IC(50) values in the range of 1.2-5.6 microg/ml.

摘要

在一个专注于藻类酚类化合物化学预防潜力的项目中，从褐藻 Fucus vesiculosus L 的乙醇提取物中获得了两种间苯三酚衍生物，属于岩藻酚类化合物，以及已知的岩藻三萜醇 A。化合物三氟二苯并间苯三酚 A 和三氟三苯并间苯三酚 A 分别由六个和七个间苯三酚单元组成。将这些化合物与单体间苯三酚一起检查其癌症化学预防潜力。三氟二苯并间苯三酚 A、三氟三苯并间苯三酚 A 和岩藻三萜醇 A 被鉴定为强自由基清除剂，其清除 1,1-二苯基-2-苦基肼自由基（DPPH）的 IC 50 值在 10.0-14.4μg/ml 范围内。所有三种化合物在氧自由基吸收能力（ORAC）测定中均能有效清除过氧自由基。此外，这些化合物还显示出抑制细胞色素 P450 1A 活性的作用，IC 50 值在 17.9-33μg/ml 范围内，以及芳香酶（Cyp19）活性，IC 50 值在 1.2-5.6μg/ml 范围内。