Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 100, Taiwan.
Biomaterials. 2010 Mar;31(7):1568-77. doi: 10.1016/j.biomaterials.2009.11.048. Epub 2009 Dec 1.
It is well known that biomaterials play an important role in the regulation of adhesion and growth of a variety of cultured cell types. However, whether biomaterials are associated with the senescence of cultured cells is not known. The present work shows that the decrease of the hydrophobic property of poly (ethylene-co-vinyl alcohol) (EVAL) from 44 mole% to 27 mole% ethylene could induce characteristic senescence-associated phenotypic changes such as larger cell shape, re-organized actin cytoskeleton, lower proliferation capacity, higher levels of senescence-associated beta-galactosidase (SA beta-gal) activity, and upregulation of the cell-cycle inhibitor p53 and its transcriptional target p21 in the cultured human diploid fibroblasts (HDFs). Furthermore, it was found that the cultured cells recovered their ability to grow when the substrate was reused every passage. It seemed that the extracellular matrix (ECM) proteins adsorbed onto the EVAL surface might have a protective role in the cellular aging process. Therefore, whether a biomaterial strongly influences cellular aging process must be considered in the selection of a biomaterial for the biomedical application.
众所周知，生物材料在调节各种培养细胞类型的黏附和生长方面起着重要作用。然而，生物材料是否与培养细胞的衰老有关尚不清楚。本工作表明，聚（乙烯-co-乙烯醇）（EVAL）中乙烯摩尔含量从 44%降低至 27%，可诱导培养的人二倍体成纤维细胞（HDF）出现典型的衰老相关表型变化，如细胞形状增大、肌动蛋白细胞骨架重新排列、增殖能力降低、衰老相关β-半乳糖苷酶（SAβ-gal）活性升高，以及细胞周期抑制剂 p53及其转录靶标 p21 的上调。此外，还发现当每隔一代重复使用基底时，培养细胞恢复了生长能力。似乎吸附在 EVAL 表面的细胞外基质（ECM）蛋白在细胞衰老过程中可能具有保护作用。因此，在选择用于生物医学应用的生物材料时，必须考虑生物材料是否会强烈影响细胞衰老过程。
