Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland.
J Rheumatol. 2010 Jan;37(1):53-9. doi: 10.3899/jrheum.090424. Epub 2009 Dec 1.
CD4+ T cells from patients with systemic lupus erythematosus (SLE) display defective function that contributes to abnormal activation of B cells and autoantibody production.
We compared the transcript and protein levels of Fyn and CD70 in CD4+ T cells from patients with SLE (n = 41) and healthy individuals (n = 34). The CD4+ T cells were isolated by positive biomagnetic separation technique. The quantitative analysis of messenger RNA was performed by reverse transcription and real-time quantitative PCR. The protein contents in the CD4+ T cells were determined by Western blotting analysis.
We observed significantly higher levels of Fyn (p = 0.03) and CD70 (p = 0.029) transcripts in SLE CD4+ T cells than in controls. There was a significant increase in CD70 protein levels (p < 0.0001), but not Fyn protein levels (p = 0.081) in CD4+ T cells from patients with SLE compared to healthy individuals. In the group with high disease activity [SLE Disease Activity Index (SLEDAI) >/= 9], we observed a significantly higher Fyn protein content than in controls (p = 0.030). There was no correlation between Fyn and CD70 protein levels in SLE CD4+ T cells and disease activity as expressed in the SLEDAI scale.
We confirmed previous observations of higher expression of CD70 in CD4+ T cells from patients with SLE. Our findings suggest that increased Fyn protein content in CD4+ T cells can be associated with high SLE disease activity.
红斑狼疮患者的 CD4+T 细胞表现出功能缺陷，这导致 B 细胞异常激活和自身抗体产生。
我们比较了 41 例系统性红斑狼疮（SLE）患者和 34 名健康个体的 CD4+T 细胞中的 Fyn 和 CD70 的转录物和蛋白水平。通过正磁珠分离技术分离 CD4+T 细胞。通过逆转录和实时定量 PCR 进行信使 RNA 的定量分析。通过 Western 印迹分析确定 CD4+T 细胞中的蛋白含量。
我们观察到 SLE CD4+T 细胞中的 Fyn（p = 0.03）和 CD70（p = 0.029）转录物水平明显高于对照组。与健康个体相比，SLE 患者的 CD4+T 细胞中 CD70 蛋白水平显著增加（p < 0.0001），但 Fyn 蛋白水平没有增加（p = 0.081）。在疾病活动度高的组（SLE 疾病活动指数[SLEDAI]>/=9）中，我们观察到 Fyn 蛋白含量明显高于对照组（p = 0.030）。SLE CD4+T 细胞中 Fyn 和 CD70 蛋白水平与 SLEDAI 量表中表达的疾病活动度之间没有相关性。
我们证实了先前观察到的 SLE 患者 CD4+T 细胞中 CD70 表达较高的现象。我们的研究结果表明，CD4+T 细胞中 Fyn 蛋白含量的增加可能与 SLE 疾病活动度高有关。
